ING1 antibody (AA 285-296)

Catalog No. ABIN290441

The Goat Polyclonal anti-ING1 antibody is suitable to detect ING1 in samples from Human. It has been validated for WB, ELISA, IHC, IF and IHC (p).

Quick Overview for ING1 antibody (AA 285-296) (ABIN290441)

Target: ING1 (Inhibitor of Growth Family, Member 1 (ING1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ING1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ING1 Antibody

Binding Specificity: AA 285-296

Specificity: Aa 285-296 of Human p33 ING1 protein (Inhibitor of growth family, member 1). This sequence shows 100 % homology to all splice variants for ING1.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from human ING1.

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ELISA (1:4000 - 1:14000), IF, IHC, IHC-P (2.5 μg/mL), WB (1:500 - 1:3000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for ING1

Target: ING1 (Inhibitor of Growth Family, Member 1 (ING1))

Alternative Name: ING1

Background: Name/Gene ID: ING1

Synonyms: ING1, Growth inhibitor ING1, Growth inhibitor p33ing1, Inhibitor of growth protein 1, p33, p33ING1, p47ING1a, Tumor suppressor ING1, p33ING1b, Growth inhibitory protein ING1, Inhibitor of growth 1, p24ING1c

Gene ID: 3621

UniProt: Q9UK53

Pathways: Protein targeting to Nucleus, Autophagy