TFAP2A antibody (AA 3-14)

Catalog No. ABIN290449

This Goat Polyclonal antibody specifically detects TFAP2A in WB, ELISA, IHC, IF and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Dog and Chicken.

Quick Overview for TFAP2A antibody (AA 3-14) (ABIN290449)

Target: TFAP2A (Transcription Factor AP-2 alpha (Activating Enhancer Binding Protein 2 Alpha) (TFAP2A))

Reactivity: Human, Mouse, Rat, Dog, Chicken

Host: Goat

Clonality: Polyclonal

Conjugate: This TFAP2A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-TFAP2A Antibody

Binding Specificity: AA 3-14

Specificity: Aa 3-14 of Human AP2A1 and AP2A2 proteins.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from human TFAP2A / AP2 Alpha.

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ELISA (1:5000 - 1:20000), IF, IHC, IHC-P (2.5 μg/mL), WB (1:500 - 1:2000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for TFAP2A

Target: TFAP2A (Transcription Factor AP-2 alpha (Activating Enhancer Binding Protein 2 Alpha) (TFAP2A))

Alternative Name: TFAP2A / AP-2

Background: Name/Gene ID: TFAP2A

Synonyms: TFAP2A, Activator protein 2, AP-2, AP-2a, AP2TF, AP-2alpha, BOFS, TFAP2, Transcription factor AP-2, AP-2 transcription factor, AP2-alpha

Gene ID: 7020

UniProt: P05549

Pathways: Caspase Cascade in Apoptosis, EGFR Signaling Pathway, Response to Water Deprivation, Sensory Perception of Sound, Tube Formation, Embryonic Body Morphogenesis, Brown Fat Cell Differentiation, Lipid Metabolism