TFAP2A antibody (C-Term)

Catalog No. ABIN6259960

The Rabbit Polyclonal anti-TFAP2A antibody (ABIN6259960) specifically detects TFAP2A in WB, ELISA, IHC, IF, ICC, EMSA and ChIP. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for TFAP2A antibody (C-Term) (ABIN6259960)

Target: TFAP2A (Transcription Factor AP-2 alpha (Activating Enhancer Binding Protein 2 Alpha) (TFAP2A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TFAP2A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Electrophoretic Mobility-Shift Assay (EMSA), Chromatin Immunoprecipitation (ChIP)

Product Details anti-TFAP2A Antibody

Binding Specificity: C-Term

Specificity: AP2 alpha Antibody detects endogenous levels of total AP2 alpha.

Predicted Reactivity: Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human AP2 alpha, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, CHIP,EMSA, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for TFAP2A

Target: TFAP2A (Transcription Factor AP-2 alpha (Activating Enhancer Binding Protein 2 Alpha) (TFAP2A))

Alternative Name: TFAP2A

Background:

Description: Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-alpha is the only AP-2 protein required for early morphogenesis of the lens vesicle. Together with the CITED2 coactivator, stimulates the PITX2 P1 promoter transcription activation. Associates with chromatin to the PITX2 P1 promoter region.

Gene: TFAP2A

Molecular Weight: 48 kDa

Gene ID: 7020

UniProt: P05549

Pathways: Caspase Cascade in Apoptosis, EGFR Signaling Pathway, Response to Water Deprivation, Sensory Perception of Sound, Tube Formation, Embryonic Body Morphogenesis, Brown Fat Cell Differentiation, Lipid Metabolism