DNMT1 antibody (AA 177-550)

Catalog No. ABIN290479

This Chicken Polyclonal antibody specifically detects DNMT1 in WB, IHC and IHC (p). It exhibits reactivity toward Human.

Quick Overview for DNMT1 antibody (AA 177-550) (ABIN290479)

Target: DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))

Reactivity: Human

Host: Chicken

Clonality: Polyclonal

Conjugate: This DNMT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-DNMT1 Antibody

Binding Specificity: AA 177-550

Specificity: Amino acids 177-550 human DNMT1

Purification: Immunoaffinity purified

Immunogen: Recombinant human DNMT1.

Type of Immunogen: Recombinant protein

Isotype: IgY

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), WB (1:2000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS. No preservative added.

Preservative: Without preservative

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for DNMT1

Target: DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))

Alternative Name: DNMT / DNMT1

Background: Name/Gene ID: DNMT1
Family: C5-methyltransferase

Synonyms: DNMT1, AIM, CXXC finger protein 9, CXXC9, DNA methyltransferase 1, DNMT, DNA methyltransferase HsaI, DNA MTase HsaI, HSN1E, M.HsaI, MCMT

Gene ID: 1786

UniProt: P26358

Pathways: SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection