SCN5A antibody (AA 1548-1558)

Catalog No. ABIN290512

This Goat Polyclonal antibody specifically detects SCN5A in IHC, ELISA and IHC (p). It exhibits reactivity toward Human.

Quick Overview for SCN5A antibody (AA 1548-1558) (ABIN290512)

Target: SCN5A (Sodium Channel, Voltage-Gated, Type V, alpha Subunit (SCN5A))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SCN5A antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SCN5A Antibody

Binding Specificity: AA 1548-1558

Specificity: Human SCN5A / Nav1.5. This antibody is expected to recognise all reported isoforms (NP_932173.1, NP_000326.2, NP_001092874.1, NP_001092875.1).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%) Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bat, Bovine, Dog, Horse, Pig, Opossum (91%) Turkey, Chicken, Pufferfish, Slime mold (82%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-ETDDQSPEKIN from an internal region of human SCN5A / Nav1.5 (NP_932173.1, NP_000326.2, NP_001092874.1, NP_001092875.1, NP_001153632.1, NP_001153633.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%), Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bat, Bovine, Dog, Horse, Pig, Opossum (91%), Turkey, Chicken, Pufferfish, Slime mold (82%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 - 10 μg/mL), Peptide-ELISA (1:32000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5-10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for SCN5A

Target: SCN5A (Sodium Channel, Voltage-Gated, Type V, alpha Subunit (SCN5A))

Alternative Name: SCN5A / Nav1.5

Background: Name/Gene ID: SCN5A
Subfamily: Sodium channel - voltage-gated
Family: Ion Channel

Synonyms: SCN5A, CDCD2, CMPD2, HB1, HB2, HH1, HBBD, Nav1.5, IVF, RSKM2X, SCAL, Scn2x, LQT3, SSS1, MH1, VF1, PFHB1, RATRSKM2X, SkM2, CMD1E, ICCD, RH1, RSkM2

Gene ID: 6331

NCBI Accession: NP_932173, NP_000326, NP_001092874, NP_001092875, NP_001153632, NP_001153633

UniProt: Q14524