WNT2B antibody

Catalog No. ABIN293249

Product Details anti-WNT2B Antibody

Target: WNT2B (Wingless-Type MMTV Integration Site Family, Member 2B (WNT2B))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This WNT2B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Specificity: Selected for its ability to recognize mouse and human Wnt-2b in the applications listed.

Purification: Affinity purified

Immunogen: E. coli-derived rmWnt-2b.

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, ICC, WB

Usage: Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Wnt-2b. The detection limit for rmWnt-2b is approximately 5 ng/lane and 1 ng/lane under non-reducing and reducing conditions, respectively. In this format, this antibody shows approximately 20 % cross-reactivity with rhWnt-2, and less than 5 % cross-reactivity with rhWnt-7a, -9a, rmWnt-1, -3a, -4, -5a, -5b, -8a, -8b, -9b, -10a, -10b, -11, -16. Immunocytochemistry: This antibody has been used at a concentration of 10 μg/mL to detect Wnt-2b in BT20 human breast carcinoma cells. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by NorthernLights-557-coupled anti-goat IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing BSA. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect mouse Wnt-2b. The detection limit for rmWnt-2b is approximately 0.5 ng/well.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: deionized water

Concentration: Lot specific

Buffer: Lyophilized.

Target Details for WNT2B

Target: WNT2B (Wingless-Type MMTV Integration Site Family, Member 2B (WNT2B))

Alternative Name: WNT2B (WNT2B Products)

Synonyms: WNT13 antibody, XWNT2 antibody, wnt2b antibody, XWnt-2 antibody, Xwnt-2b antibody, wnt-2b antibody, xwnt2b antibody, WNT2B antibody, Wnt13 antibody, Wnt family member 2B antibody, wingless-type MMTV integration site family, member 2Ba antibody, Wnt family member 2B L homeolog antibody, wingless-type MMTV integration site family, member 2B antibody, wingless-type MMTV integration site family, member 2Bb antibody, WNT2B antibody, Wnt2b antibody, wnt2ba antibody, wnt2b.L antibody, wnt2bb antibody

Background: Name/Gene ID: WNT2B
Family: WNT

Synonyms: WNT2B, Protein Wnt-2b, XWNT2, Protein Wnt-13, WNT13, XWNT2, Xenopus, homolog of

Gene ID: 7482

UniProt: Q93097

Pathways: WNT Signaling