TNFRSF11A antibody (AA 326-616)

Catalog No. ABIN296494

This Mouse Monoclonal antibody specifically detects TNFRSF11A in WB, IHC, FACS and IHC (p). It exhibits reactivity toward Human and Mouse.

Quick Overview for TNFRSF11A antibody (AA 326-616) (ABIN296494)

Target: TNFRSF11A (Tumor Necrosis Factor Receptor Superfamily, Member 11a, NFKB Activator (TNFRSF11A))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This TNFRSF11A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 9A725

Product Details anti-TNFRSF11A Antibody

Binding Specificity: AA 326-616

Specificity: In RAW cells, a 97 kD is observed.

Purification: Protein G purified

Immunogen: A fusion protein containing amino acid residues 326-616 of human RANK.

Type of Immunogen: Fusion protein

Isotype: IgG1

Application Details

Application Notes: Approved: Flo, IHC, IHC-P (10 μg/mL), WB (1 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS containing 0.5 % BSA, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for TNFRSF11A

Target: TNFRSF11A (Tumor Necrosis Factor Receptor Superfamily, Member 11a, NFKB Activator (TNFRSF11A))

Alternative Name: TNFRSF11A / RANK

Background: Name/Gene ID: TNFRSF11A
Family: TNF Receptor

Synonyms: TNFRSF11A, CD265, CD265 antigen, ODFR, LOH18CR1, OPTB7, OSTS, PDB2, RANK, Receptor activator of NF-KB, TRANCER, FEO, OFE

Gene ID: 8792

UniProt: Q9Y6Q6

Pathways: NF-kappaB Signaling