Sca-1/Ly-6A/E antibody

Catalog No. ABIN299003

The Rat Monoclonal anti-Sca-1/Ly-6A/E antibody is suitable to detect Sca-1/Ly-6A/E in samples from Mouse. It has been validated for FACS, WB and IP.

Quick Overview for Sca-1/Ly-6A/E antibody (ABIN299003)

Target: Sca-1/Ly-6A/E (Ly6a) (Lymphocyte Antigen 6 Complex, Locus A (Ly6a))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Sca-1/Ly-6A/E antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP)

Clone: D7

Product Details anti-Sca-1/Ly-6A/E Antibody

Specificity: Recognizes members of the lymphocyte activation protein-6 (Ly6) multigene family. Specifically recognizes Ly6A.2 and Ly6E.1. Ly6A/E is also known as stem cell antigen-1 (Sca-1). It is an 18kD mouse glycosyl phosphatidylinositol-anchored cell surface protein of the Ly6 gene family. It is expressed on multipotent hematopoietic stem cells in bone marrow of mice with both the Ly6.1 and Ly6.2 allotypes. As such, it is the most common marker used for enriching adult hematopoietic stem cells. It has varied functions, it plays a role in bone formation, myoblast proliferation and differentiation and the activation and immunosuppressive function of T cells.

Purification: Purified

Immunogen: IL-2-dependent mouse T cell line CTL-L.

Type of Immunogen: Cells

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Borate buffered saline,  pH 8.0, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Short term: -20°C
Long term: -20°C.

Target Details for Sca-1/Ly-6A/E

Target: Sca-1/Ly-6A/E (Ly6a) (Lymphocyte Antigen 6 Complex, Locus A (Ly6a))

Alternative Name: Ly 6A/E / Sca-1

Pathways: Sensory Perception of Sound, Activated T Cell Proliferation