Goat anti-Human IgG (F(ab')2 Region) Antibody (HRP)
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- Target See all IgG products
- IgG
- Fragment
- F(ab')2 fragment
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Binding Specificity
- F(ab')2 Region
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Reactivity
- Human
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- HRP
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Application
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- Specificity
- Anti-Peroxidase, anti-Goat Serum, Human IgG, Human IgG F(ab')2 and Human Serum. No reaction was observed against anti- Pepsin, anti-Goat IgG F(c), Human IgG F(c) or Bovine, Horse, Mouse and Rat Serum Proteins
- Purification
- Immunoaffinity purified
- Immunogen
- Human IgG F(ab')2 fragment.
- Isotype
- IgG
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- Application Notes
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Approved: ELISA (1:10000 - 1:40000), IHC (1:500 - 1:2500), WB (1:1000 - 1:5000)
Usage: Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- deionized water
- Concentration
- Lot specific
- Buffer
- Lyophilized from 0.02M Potassium Phosphate, 0.15M NaCl, pH 7.2 with 10 mg/mL BSA( IgG and Protease free) and 0.01 % Gentamicin Sulfate. Restore with 2.0 mL D water
- Preservative
- Gentamicin sulfate
- Precaution of Use
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
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Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase. - Storage
- 4 °C,-20 °C
- Storage Comment
- Store lyophilized at 4°C. Following resuspension aliquot and store at -20°C. Avoid freeze-thaw cycles and dilute immediately before use.
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- Target
- IgG
- Abstract
- IgG Products
- Target Type
- Antibody
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