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Goat anti-Human IgG (F(ab')2 Region) Antibody (HRP)

ELISA, IHC, WB Polyclonal IgG HRP F(ab')2 fragment
Catalog No. ABIN301338
  • Target See all IgG products
    IgG
    Fragment
    F(ab')2 fragment
    Binding Specificity
    • 2356
    • 502
    • 411
    • 188
    • 130
    • 123
    • 94
    • 29
    • 28
    • 28
    • 17
    • 9
    • 6
    • 6
    • 6
    • 6
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    F(ab')2 Region
    Reactivity
    • 697
    • 680
    • 568
    • 452
    • 368
    • 239
    • 200
    • 148
    • 140
    • 132
    • 122
    • 98
    • 96
    • 95
    • 94
    • 63
    • 46
    • 33
    • 27
    • 16
    • 12
    • 10
    • 8
    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 1727
    • 1486
    • 537
    • 216
    • 118
    • 103
    • 33
    • 31
    • 21
    • 17
    • 15
    • 11
    • 11
    • 1
    Goat
    Clonality
    • 3354
    • 193
    • 1
    Polyclonal
    Conjugate
    • 587
    • 410
    • 408
    • 369
    • 297
    • 276
    • 228
    • 120
    • 99
    • 67
    • 66
    • 62
    • 62
    • 52
    • 48
    • 47
    • 44
    • 43
    • 43
    • 41
    • 39
    • 32
    • 32
    • 32
    • 31
    • 30
    • 29
    • 29
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 27
    • 26
    • 26
    • 25
    • 21
    • 19
    • 17
    • 17
    • 16
    • 16
    • 15
    • 15
    • 14
    • 10
    HRP
    Application
    • 1867
    • 1788
    • 1785
    • 1535
    • 988
    • 934
    • 913
    • 515
    • 234
    • 115
    • 115
    • 110
    • 100
    • 87
    • 77
    • 50
    • 50
    • 49
    • 32
    • 27
    • 25
    • 21
    • 17
    • 16
    • 11
    • 8
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    Specificity
    Anti-Peroxidase, anti-Goat Serum, Human IgG, Human IgG F(ab')2 and Human Serum. No reaction was observed against anti- Pepsin, anti-Goat IgG F(c), Human IgG F(c) or Bovine, Horse, Mouse and Rat Serum Proteins
    Purification
    Immunoaffinity purified
    Immunogen
    Human IgG F(ab')2 fragment.
    Isotype
    IgG
  • Application Notes
    Approved: ELISA (1:10000 - 1:40000), IHC (1:500 - 1:2500), WB (1:1000 - 1:5000)

    Usage: Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
    Comment

    Target Species of Antibody: Human

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    deionized water
    Concentration
    Lot specific
    Buffer
    Lyophilized from 0.02M Potassium Phosphate, 0.15M NaCl,  pH 7.2 with 10 mg/mL BSA( IgG and Protease free) and 0.01 % Gentamicin Sulfate. Restore with 2.0 mL D water
    Preservative
    Gentamicin sulfate
    Precaution of Use
    This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing. Dilute only prior to immediate use
    Centrifuge product if not completely clear after standing at room temperature.
    Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store lyophilized at 4°C. Following resuspension aliquot and store at -20°C. Avoid freeze-thaw cycles and dilute immediately before use.
  • Target
    IgG
    Abstract
    IgG Products
    Target Type
    Antibody
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