Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4) antibody (PE)

Antigen: Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4)

Synonyms: LeX, CD15, ELFT, FCT3A, FUTIV, SSEA-1, FUC-TIV

Clonality: Monoclonal (MEM-158)

Host: Mouse

Reactivity: Human

Conjugate: PE

Application: Flow Cytometry (FACS)

Catalog no.: ABIN302030

Additional Information

Alternative name: CD15

Immunogen: Human granulocytes

Isotype: IgM

Clone: MEM-158

Description: CD15 (Lewis X, Le(x), stage specific embryonic antigen-1, SSEA-1) is a trisacharidedeterminant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteinsand proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes,macrophages, cells of gastric mucosa, nervous system or various tumour cells. There areseveral variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with highsurface expression of Le(x) antigen exhibit strong self-aggregation, based oncalcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryocompaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomersialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirmdiagnosis of Hodgkin's disease.

Specificity: The antibody MEM-158 reacts with CD15, a cell membrane molecule3-fucosyl-N-acetyllactosamine (3-FAL) strongly expressed on granulocytes, monocytes,macrophages, mast cells, it is also present on Langerhans cells and some myeloidprecursors cells.HLDA VI, WS Code AS A053 This antibody does not react with: Porcine .

Application Details

Application Notes: The reagent is designed for Flow Cytometry analysis of human blood cells using 20 µl reagent / 100 µl of whole blood or 10 6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

Buffer: The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azideand 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent. Preparation: The purified antibody is conjugated with R-Phycoerythrin (PE)

Storage: Store in the dark at 2-8 o C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.Short-term exposure to room temperature should not affect the quality of the reagent.However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.

Research Area: Stem Cells, Hematopoietic Progenitors, Hematopoietic Stem Cells, CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only

Images

Surface staining of human peripheral blood cells with anti-human CD15 (MEM-158) FITC. Cells in the granulocyte gate were used for analysis.