Linker For Activation of T Cells (LAT) antibody

Antigen: Linker For Activation of T Cells (LAT)

Synonyms: LAT1, pp36, MGC108666, LAT, MGC157208, lat

Clonality: Monoclonal (LAT-01)

Host: Mouse

Reactivity: Human

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN302069

Additional Information

Alternative name: LAT

Immunogen: Bacterially produced recombinant polypeptide corresponding to the entire cytoplasmicdomain of human LAT.

Format: Purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: LAT-01

Description: LAT (Linker for Activation of T cells) is a 36-38 kDa double-palmitoylated transmembraneadaptor protein expressed by T cells, pre-B cells, NK cells, mast cells and platelets. Afterimmunoreceptor triggering, LAT becomes multiply tyrosine-phosphorylated by Syk-, Src-, orTec-family kinases, providing docking sites for downstream signaling molecules. LAT isessential for TCR-dependent T cell- and FcepsilonRI-dependent mast cell activation, as wellas for maturation of early thymocytes. It is also involved in NK cell signaling and plateletactivation.

Specificity: The antibody LAT-01 reacts with Linker for activation of T cells (LAT), a 36-38 kDatransmembrane adaptor protein expressed by T cells, pre-B cells, NK cells, mast cells andplatelets. This antibody does not react with: Mouse, Other not tested.

Application Details

Application Notes: Western BlottingImmunoprecipitation. Application note: Tyrosine-phosphorylated form of LAT is not optimally recognized. Flow Cytometry

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from hybridoma culture supernatant by protein A-affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Research Area: Signaling, Adaptive Immunity

Restrictions: For Research Use only

Images

Flow Cytometry analysis