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PKAc antibody

Antigen

PKAc
Clonality Monoclonal (6D2-1)
Host

Mouse
Reactivity

Human
Application
Western Blotting (WB)
Catalog no. ABIN302080
Quantity 0.1 mg  (1 mg/ml)
Price 286.00 $   Plus shipping costs $35.00
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anti-PKAc antibody

Additional Information
Application Details Images Secondary Antibodies back to top

Immunogen Peptide corresponding to amino acids ESPAQNTAHLDQFERIK of human proteinkinase A calpha (PKAc alpha).
Format Purified
Isotype IgG1  (Matching secondary antibodies)
Clone 6D2-1
Description Protein kinase A (PKA, cAMP-dependent protein kinase) is a key element of a ubiquitoussignaling pathway important in the cell cycle, cellular communication, memory formation andbehavior. PKA is composed of two catalytic (PKAc, Protein Kinase A catalytic subunit) andtwo regulatory subunits (PKAr). Upon binding cAMP, the complex dissociates to PKAr dimerand two activated PKAc ser/thr protein kinase catalytic monomers. The released PKAc cantranslocate into the nucleus and exert a regulatory role in the activation of multiple nuclearhormone receptors. However, PKAc-mediated activation of tonicity-dependent geneexpression is cAMP independent. Humans express three types of PKAc subunit PKAc alphais present in most human tissues, PKAc beta and gamma are tissue-specific, the later isfound in testes.
Specificity The antibody 6D2.1 strongly reacts with human proteinkinase A catalytic (PKAc) alphasubunit, and weakly with PKAc gamma subunit (both around 40 kDa). The recognizedepitope of PKAc alpha is identical between man, sheep, pig, ox and dog.

Application Details
Images Secondary Antibodies back to top

Application Notes Western Blotting - Recommended dilution: 0.5-1 µg/ml. Positive control: HeLa human cervix carcinoma cell line. Application note: To detect PKAc gamma, use a more concentrated lysate from a tissueexpressing this subunit (testis).
Concentration 1 mg/ml
Purity > 95% (by SDS-PAGE)
Purification Purified from cell culture supernatant by protein-A affinity chromatography.
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Storage Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.
Restrictions For Research Use only