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Cyclin D1 (CCND1) antibody

Antigen

Cyclin D1 (CCND1)
Synonyms
BCL1, PRAD1, U21B31, D11S287E, cD1, Cyl-1, bcl-1, AI327039, DIP1, GCIP, Maid, SECC-8, SSEC-8, AU022347, cb161, cycd1, fb52e01, fc83a12, id:ibd1198, wu:fb52e01, wu:fc83a12, MGC109078, ccnd1b, MGC52909, ... show more
Clonality Monoclonal (CD1-1)
Host
Alternatives

Mouse
Reactivity
Alternatives

Human, Rat (Rattus)
Application
Alternatives Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Immunocytochemistry (ICC)
Catalog no. ABIN302101
Quantity 0.1 mg  (1 mg/ml)
Price 325.00 $   Plus shipping costs $35.00
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Additional Information
Application Details Alternatives Secondary Antibodies back to top

Alternative name Cyclin D1
Immunogen Purified cyclin D1 protein
Format Purified
Isotype IgG1  (Matching secondary antibodies)
Clone CD1-1
Description Cyclin D1 (PRAD1, Bcl-1) is a cytoplasmic and nuclear protein, which is synthesized duringG1 phase and assembles with either cyclin-dependent kinase 4 (CDK4) or CDK6 inresponse to growth factor stimulation. D-type cyclin-CDK complexes act to inactivate thegrowth-suppressive function of the Rb protein through its phosphorylation, and titrate CDKinhibitors such as p21Cip1 and p27Kip1. Whereas during G1 phase cyclin D1 accumulatesin the nucleus, it translocates into the cytoplasm during S phase. Without growthfactor-mediated stimulation cyclin D1 is unstable, and undergoes ubiquitin-mediateddegradation, which is triggered by its phosphorylation. Cyclin D1 destabilization participatesin G1/S phase arrest.
Specificity The antibody CD1.1 recognizes cyclin D1, an ubiquitously expressed 33 kDa protein thatmigrates as a 36 kDa band under reducing SDS-PAGE conditions.
Synonyms BCL1, PRAD1, U21B31, D11S287E, cD1, Cyl-1, bcl-1, AI327039, DIP1, GCIP, Maid, SECC-8, SSEC-8, AU022347, cb161, cycd1, fb52e01, fc83a12, id:ibd1198, wu:fb52e01, wu:fc83a12, MGC109078, ccnd1b, MGC52909, MGC132138, ccnd1, MGC53090, bcl1, prad1, u21b31, MGC69515, d11s287e, CCND1, ccndbp1, MGC137744, ZHM, CCNDBP1, MGC133757, DKFZp468E2216, DKFZp469C1216, DKFZp469H0317

Application Details
Alternatives Secondary Antibodies back to top

Application Notes Flow Cytometry. Application note: Membrane permeabilization is required. Immunoprecipitation - Recommended dilution: 1 µg/ml. - Recommended dilution: 1 µg/ml. Immunohistochemistry (paraffin sections) - Pretreatment: Heat treatment, sodium citrate buffer pH 6. 0.Immunohistochemistry (frozen sections) - Recommended dilution: 2 µg/ml. Positive tissue: colon. Immunocytochemistry - Recommended dilution: 1 µg/ml
Concentration 1 mg/ml
Purity > 95% (by SDS-PAGE)
Purification Purified from ascites by protein-A affinity chromatography.
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Storage Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.
Research Area Cancer, Cell Cycle
Restrictions For Research Use only