GTPase NRas (NRAS) (Internal Region) antibody

Details for Product No. ABIN302138
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Synonyms NRAS, AV095280, N-ras, ALPS4, NRAS1, NS6, N-RAS, fj99a10, wu:fj99a10, zgc:100761, zras-b1, H-RAS, HRAS
Internal Region
(7), (7), (6), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1)
Human, Rat (Rattus)
(59), (9), (8), (4), (1), (1)
(27), (19), (14)
(1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(59), (32), (18), (12), (7), (6), (5), (4), (1), (1)
Pubmed 1 reference available
Quantity 100 μg
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Catalog No. ABIN302138
325.97 $
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Immunogen Peptide with sequence C-RMKKLNSSDDGTQ, from the internal region of the protein sequence according to NP_002515.1.
Cross-Reactivity Mouse (Murine), Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name NRAS
Background Synonyms: NRAS, neuroblastoma RAS viral (v-ras) oncogene homolog, N-ras, NRAS1, N-ras protein part 4, v-ras neuroblastoma RAS viral oncogene homolog
Gene ID 4893, 18176, 24605
NCBI Accession NP_002515
Research Area Cancer
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx. 21 kDa band observed in Human lysates of cell line A431 and of Rat Intestines (calculated MW of 21.2 kDa according to NP_002515.1). In transfected HEK293 transiently expressing NRAS bands of approx. 21 kDa and 25 kDa are observed. These bands are not observed in the non-transfected HEK293. Recommended concentration: 0.01-0.03 µg/mL.
IHC: In paraffin embedded Human Small Intestine shows staining of cytoplasm and membrane of enterocytes. Recommended concentration: 2-3 µg/mL.
Restrictions For Research Use only
Format Liquid
Concentration 100µg specific antibody in 200µl
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-GTPase NRas (NRAS) (Internal Region) antibody EB08365 (2.5µg/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
General Thomas, Edmiston, Alexander et al.: "Number of nevi and early-life ambient UV exposure are associated with BRAF-mutant melanoma." in: Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, Vol. 16, Issue 5, pp. 991-7, 2007 (PubMed).

Validation Images
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