A Kinase (PRKA) Anchor Protein 10 (AKAP10) antibody

Antigen: A Kinase (PRKA) Anchor Protein 10 (AKAP10)

Synonyms: PRKA10, D-AKAP2, MGC9414, 1500031L16Rik, B130049N18Rik, D-akap2, MGC84612, AKAP10, MGC148842, fc10g11, wu:fc10g11, si:dkey-197m14.4

Clonality: Polyclonal

Host: Goat

Reactivity: Rat (Rattus)

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN302151

Additional Information

Alternative name: AKAP10

Gene ID: 11216, 56697 (mouse), 360540 (rat)

Immunogen: C-RKVKGKEQEKTSD

Format: Affinity-purified

Description: All names and symbols: AKAP10, A kinase (PRKA) anchor protein 10, D-AKAP2, MGC9414, PRKA10, A-kinase anchor protein 10, dual-specificity A-kinase anchoring protein 2, mitochondrial A kinase PPKA anchor protein 10, protein kinase A anchoring protein 10. Official Symbol: AKAP10. RefSeq number(s): NP_009133.2

Specificity: Human, Mouse, Rat, Dog, Cow

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx. 74kDa band observed in Rat Brain lysates (calculated MW of 73.8kDa according to Human NP_009133.2 and according to Rat NP_001108078.1). Recommended concentration: 0.3-1µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Signaling

Restrictions: For Research Use only

Images

ABIN302151 (1µg/ml) staining of Rat Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Burns-Hamuro, Barraclough, Taylor: "Identification and functional analysis of dual-specific A kinase-anchoring protein-2." in: Methods in enzymology, Vol. 390, pp. 354-74, 2004 (PubMed).