CERK antibody (Extracellular Domain, N-Term)

Catalog No. ABIN302420

The Rabbit Polyclonal anti-CERK antibody is suitable to detect CERK in samples from Human. It has been validated for IHC (p).

Quick Overview for CERK antibody (Extracellular Domain, N-Term) (ABIN302420)

Target: CERK (Ceramide Kinase (CERK))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CERK antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CERK Antibody

Binding Specificity: Extracellular Domain, N-Term

Specificity: This antibody detects Ceramide Kinase (CERK), N-terminal extracellular domain.

Purification: Immunoaffinity Chromatography

Immunogen: CERK antibody was raised against synthetic peptide from N-terminal extracellular domain of human CERK.

Application Details

Application Notes: Immunohistochemistry on paraffin sections (20 μg/mL). Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1xTBS with Tween (TBST) for 1 min. at room temperature.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % sodium azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C to -70 °C for longer.

Target Details for CERK

Target: CERK (Ceramide Kinase (CERK))

Alternative Name: Ceramide Kinase

Background: CERK, which phosphorylates Cer to produce ceramide 1-phosphate (C1P), was initially described as a Ca2+-stimulated lipid kinase that copurified with brain synaptic vesicles. CERK activity has been reported in HL60 cells, mast cells, and neutrophils. CERK is involved in phagolysosome formation in polymorphonuclear leukocytes and also in liposome fusion. C1P has been reported to have mitogenic effects. More recently, C1P was found to induce arachidonic acid release and prostanoid synthesis.Synonyms: Acylsphingosine kinase, KIAA1646, LK4, Lipid kinase 4, hCERK

Gene ID: 64781

NCBI Accession: NP_073603

UniProt: Q8TCT0