CERK antibody (C-Term)

Catalog No. ABIN391126

Product Details anti-CERK Antibody

Target: CERK (Ceramide Kinase (CERK))

Binding Specificity: AA 487-516, C-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CERK antibody is un-conjugated

Application: Western Blotting (WB)

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This CERK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 487-516 amino acids from the C-terminal region of human CERK.

Clone: RB5271

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-CERK antibody (ABIN391126)

Murakami, Ito, Hagiwara, Yoshida, Sobue, Ichihara, Takagi, Kojima, Tanaka, Tamiya-Koizumi, Kyogashima, Suzuki, Banno, Nozawa, Murate: "ATRA inhibits ceramide kinase transcription in a human neuroblastoma cell line, SH-SY5Y cells: the role of COUP-TFI." in: Journal of neurochemistry, Vol. 112, Issue 2, pp. 511-20, (2010) (PubMed).

Yang, Gagarin, St Laurent, Hammell, Toma, Hu, Iwasa, McCaffrey: "Cardiovascular inflammation and lesion cell apoptosis: a novel connection via the interferon-inducible immunoproteasome." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 29, Issue 8, pp. 1213-9, (2009) (PubMed).

Hinkovska-Galcheva, Clark, VanWay, Huang, Hiraoka, Abe, Borofsky, Kunkel, Shanley, Shayman, Lanni, Petty, Boxer: "Ceramide kinase promotes Ca2+ signaling near IgG-opsonized targets and enhances phagolysosomal fusion in COS-1 cells." in: Journal of lipid research, Vol. 49, Issue 3, pp. 531-42, (2008) (PubMed).

Target Details for CERK

Target: CERK (Ceramide Kinase (CERK))

Alternative Name: CERK (CERK Products)

Background: Ceramide kinases convert the sphingolipid metabolite ceramide into ceramide-1-phosphate, both key mediators of cellular apoptosis and survival. Ceramide metabolism plays an essential role in the viability of neuronal cells, the membranes of which are particularly rich in sphingolipids. CERK catalyzes specifically the phosphorylation of ceramide to form ceramide 1-phosphate. This enzyme acts efficiently on natural and analog ceramides (C6, C8, C16 ceramides, and C8 dihydroceramide), and to a lesser extent on C2-ceramide and C6-dihydroceramide, but not on other lipids, such as various sphingosines. High level expression is noted in heart, brain, skeletal muscle, kidney and liver, moderate expression in peripheral blood leukocytes and thymus, and low expression in spleen, small intestine, placenta and lung.

Gene ID: 64781

NCBI Accession: NP_073603

UniProt: Q8TCT0