PLA2G3 antibody (Internal Region)

Catalog No. ABIN302624

Product Details anti-PLA2G3 Antibody

Target: PLA2G3 (Phospholipase A2, Group III (PLA2G3))

Binding Specificity: Internal Region

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PLA2G3 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects Group III Secreted Phospholipase A2 (GIII-SPLA2) (PLA2G3) at internal domain.

Purification: Immunoaffinity chromatography

Immunogen: Synthetic peptide - KLH conjugated, internal domain of human

Application Details

Application Notes: Immunohistochemistry on paraffin sections (2.5 μg/mL). Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1xTBS with Tween (TBST) for 1 min. at room temperature.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline containing 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 °C to 8 °C up to one month or (in aliquots) at -20 °C to -70 °C for longer.

Target Details for PLA2G3

Target: PLA2G3 (Phospholipase A2, Group III (PLA2G3))

Alternative Name: PLA2G3 / sPLA2-III (PLA2G3 Products)

Synonyms: PLA2G3 antibody, GIII-SPLA2 antibody, SPLA2III antibody, 9130003P18Rik antibody, phospholipase A2 group III antibody, phospholipase A2, group III antibody, PLA2G3 antibody, Pla2g3 antibody

Background: Human group III secreted phospholipase A2 belongs to a superfamily of intracellular and secreted enzymes that catalyze the hydrolysis of glycerophospholipids at the sn-2 position to release fatty acids and lysophospholipids. Group III PLA2 enzymes were originally identified in invertebrates such as bees and scorpions. Human group III sPLA2 has been shown to be a calcium dependent enzyme. It consists of a central group III sPLA2 domain flanked by unique N- and C-terminal domains that are proteolytically removed in most tissues. The sPLA2 domain is sufficient for catalytic activity. SPLA2-III is preferentially expressed in the microvascular endothelium of tissues with inflammation, ischemic injury and cancer.Synonyms: Group 3 secretory phospholipase A2, Group III secretory phospholipase A2, Phosphatidylcholine 2-acylhydrolase GIII

Gene ID: 50487

NCBI Accession: NP_056530

UniProt: Q9NZ20