RAD52 antibody (AA 360-375)

Catalog No. ABIN303138

The Rabbit Polyclonal anti-RAD52 antibody (ABIN303138) specifically detects RAD52 in WB, IF, IHC (p) and EIA. The antibody is reactive with Human samples.

Quick Overview for RAD52 antibody (AA 360-375) (ABIN303138)

Target: RAD52 (RAD52 Homolog (S. Cerevisiae) (RAD52))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAD52 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-RAD52 Antibody

Binding Specificity: AA 360-375

Purification: Immunoaffinity Chromatography.

Immunogen: Synthetic peptide corresponding to AA 360-375 of the Human Rad 52 protein.

Application Details

Application Notes: Immunohistochemistry on Paraffin Sections (2.5 μg/mL). Immunofluorescence. Western Blot (1/500-1/2000). ELISA (1/5000-1/20000).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at-20 °C. Dilute only prior to immediate use.

Target Details for RAD52

Target: RAD52 (RAD52 Homolog (S. Cerevisiae) (RAD52))

Alternative Name: RAD52

Background: The protein encoded by this gene shares similarity with Saccharomyces cerevisiae Rad52, a protein important for DNA double-strand break repair and homologous recombination. This gene product was shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombination protein RAD51, which suggested its role in RAD51 related DNA recombination and repair. Four alternatively spliced transcript variants encoding different isoforms have been reported for this gene. The alpha variant encodes the longest isoform that contains an identical N-terminus, but a distinct C-terminus, as compared to other isoforms (beta, delta, and gamma).Synonyms: DNA repair protein RAD52 homolog

Gene ID: 5893

NCBI Accession: NP_602296

UniProt: P43351

Pathways: DNA Damage Repair