TFAP2A antibody (AA 3-14)

Catalog No. ABIN303152

The Goat Polyclonal anti-TFAP2A antibody is suitable to detect TFAP2A in samples from Human. It has been validated for WB, IF, IHC (p) and EIA.

Quick Overview for TFAP2A antibody (AA 3-14) (ABIN303152)

Target: TFAP2A (Transcription Factor AP-2 alpha (Activating Enhancer Binding Protein 2 Alpha) (TFAP2A))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This TFAP2A antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-TFAP2A Antibody

Binding Specificity: AA 3-14

Specificity: Recognizes Transcription Factor Ap-2 (TFAP2A).

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Immunoaffinity Chromatography.

Immunogen: Synthetic peptide corresponding to Amino Acids 3-14 of Human AP2A1 and AP2A2 proteins.

Application Details

Application Notes: Immunohistochemistry on Paraffin Sections (2.5 μg/mL). Immunofluorescence. Western Blot (1/500-1/2000). ELISA (1/5000-1/20000).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer. Dilute only prior to immediate use.

Target Details for TFAP2A

Target: TFAP2A (Transcription Factor AP-2 alpha (Activating Enhancer Binding Protein 2 Alpha) (TFAP2A))

Alternative Name: TFAP2A / AP-2 alpha

Background: The AP2A1 and AP2A2 represent the AP2 alpha proteins that are found in the AP2 complex in clathrin-coated vesicles. AP2A is also known as Alpha-adaptin A, Adaptor protein complex AP-2 alpha-1 subunit, Clathrin assembly protein complex 2 alpha-A large chain, 100 kDa coated vesicle protein A and Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit. The AP2 complex is a heterotetramer consisting of two large adaptins (alpha or beta), a medium adaptin (mu), and a small adaptin (sigma). The complex is part of the protein coat on the cytoplasmic face of coated vesicles, which link clathrin to receptors in vesicles.Synonyms: AP-2 transcription factor, AP2, AP2TF, Activating enhancer-binding protein 2-alpha, Activator protein 2, TFAP2

Gene ID: 7020

NCBI Accession: NP_001027451

UniProt: P05549

Pathways: Caspase Cascade in Apoptosis, EGFR Signaling Pathway, Response to Water Deprivation, Sensory Perception of Sound, Tube Formation, Embryonic Body Morphogenesis, Brown Fat Cell Differentiation, Lipid Metabolism