CASP5 antibody (Center)

Catalog No. ABIN303202

This Rabbit Polyclonal antibody specifically detects CASP5 in WB, IF and IHC (p). It exhibits reactivity toward Human.

Quick Overview for CASP5 antibody (Center) (ABIN303202)

Target: CASP5 (Caspase 5, Apoptosis-Related Cysteine Peptidase (CASP5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CASP5 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CASP5 Antibody

Binding Specificity: Center

Purification: Immunoaffinity Chromatography.

Immunogen: Synthetic peptide corresponding to 14 amino acid peptide from the center of human Caspase-5.

Application Details

Application Notes: Immunohistochemistry on Paraffin Sections (5 μg/mL). Immunocytochemistry. Western Blot (0.5-1 μg/mL).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate Buffered Saline PBS, containing 0.02 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C to -70 °C for longer. Dilute only prior to immediate use.

Target Details for CASP5

Target: CASP5 (Caspase 5, Apoptosis-Related Cysteine Peptidase (CASP5))

Alternative Name: Caspase-5

Background: Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. Members of this subfamily include caspase-1, -4, -5, and -12. Activation of these caspases results in the cleavage and activation of proinflammatory cytokines such as IL-1b and IL-18. Caspase-5 can interact with caspase-1, both are constituents of the NALP1 inflammasome, a complex that can trigger the cleavage of pro-IL-1b. Expression of caspase-5 can be regulated by lipopolysaccharide (LPS) and IFN-g.Synonyms: CASP-5, CASP5, ICE(rel)-III, ICH-3 protease, ICH3, TY protease

Gene ID: 838

NCBI Accession: NP_001129581

UniProt: P51878

Pathways: Inflammasome