SMAD3 antibody (pSer423, pSer425)

Catalog No. ABIN303328

This Rabbit Polyclonal antibody specifically detects SMAD3 in WB, IF, IHC (p) and EIA. It exhibits reactivity toward Human, Mouse, Rat, Pig, Chicken, Zebrafish (Danio rerio), Cow and Frog. It has been mentioned in 3+ publications

Quick Overview for SMAD3 antibody (pSer423, pSer425) (ABIN303328)

Target: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Reactivity: Human, Mouse, Rat, Pig, Chicken, Zebrafish (Danio rerio), Cow, Frog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-SMAD3 Antibody

Binding Specificity: AA 417-425, pSer423, pSer425

Specificity: This antibody recognises SMAD3 pSer423/425.

Cross-Reactivity (Details): Species reactivity (expected):Bovine, Chicken, Frog, Mouse, Pig, Rat and Zebrafish.
Species reactivity (tested):Human.

Purification: Immunoaffinity Chromatography.

Immunogen: Synthetic peptide corresponding to amino acids 417-425 of Human SMAD3 protein.

Application Details

Application Notes: ELISA (1/25000-1/100000). Western Blot (1/2000-1/20000). Immunofluorescence. Immunohistochemistry on Paraffin Sections (2.5 μg/mL). Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100 °C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1xTBS with Tween (TBST) for 1 min. at room temperature.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-SMAD3 antibody (ABIN303328)

Sun, Zhou, Gui, Shimokado, Nakanishi, Oikawa, Sato, Muragaki: "Elevated serum 1,25(OH)2-vitamin D3 level attenuates renal tubulointerstitial fibrosis induced by unilateral ureteral obstruction in kl/kl mice." in: Scientific reports, Vol. 4, pp. 6563, (2014) (PubMed).

Carracedo, Lu, Popova, Jonsson, Eckes, Gullberg: "The fibroblast integrin alpha11beta1 is induced in a mechanosensitive manner involving activin A and regulates myofibroblast differentiation." in: The Journal of biological chemistry, Vol. 285, Issue 14, pp. 10434-45, (2010) (PubMed).

Liu, Carson-Walter, Cooper, Winans, Johnson, Walter: "Vascular gene expression patterns are conserved in primary and metastatic brain tumors." in: Journal of neuro-oncology, Vol. 99, Issue 1, pp. 13-24, (2010) (PubMed).

Target Details for SMAD3

Target: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Alternative Name: SMAD3

Background: Smad3 (also known as Mothers against decapentaplegic homolog 3 Mothers against DPP homolog 3, Mad3, hMAD-3, JV15-2 or hSMAD3) is a transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinase. These activators exert diverse effects on a wide array of cellular processes. The Smad proteins mediate much of the signaling responses induced by the TGF-b superfamily. Briefly, activated type I receptor phosphorylates receptor-activated Smads (R-Smads) at their c-terminal two extreme serines in the SSXS motif, e.g. Smad2 and Smad3 proteins in the TGF-b pathway, or Smad1, Smad5 or Smad8 in the BMP pathway. Then the phosphorylated R-Smad translocated into nucleus, where they regulate transcription of target genes. Based on microarray and animal model experiments, Smad3 accounts for at least 80 % of all TGF-b-mediated response.Synonyms: JV15-2, MAD homolog 3, MAD-3, MADH3, Mad3, Mothers against DPP homolog 3, Mothers against decapentaplegic homolog 3, SMAD 3, SMAD family member 3, SMAD-3

Gene ID: 4088

NCBI Accession: NP_001138574

UniProt: P84022

Pathways: Cell Division Cycle, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagy