GLI Family Zinc Finger 3 (GLI3) (AA 41-57) antibody

Antigen: GLI Family Zinc Finger 3 (GLI3)

Synonyms: PHS, ACLS, GCPS, PAPA, PAPB, PAP-A, PAPA1, PPDIV, Xt, Bph, Pdn, add, AI854843, AU023367, im:7142603, GLI3

Epitope: AA 41-57

Clonality: Polyclonal

Host: Rabbit

Reactivity: Dog (Canine)

Application: Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN303380

Additional Information

Characteristics: Synonyms: GLI-3, Zinc finger protein GLI3

Alternative name: GLI3

Swiss-Prot: P10071

Immunogen: Synthetic Peptide -KLH conjugated corresponding to Amino acids 41-57 of Human Gli-3protein. Remarks: Antigen Modification: aa 41-57

Cross-Reactivity: Dog (Canine), Chicken, Human, Monkey, Rat (Rattus)

Description: Gli-3 (also known as Zinc Finger Protein Gli-3 or GLI-Kruppel family member GLI-3) belongsto the GLI C2H2-type zinc-finger protein family and contains 5 C2H2-type zinc fingers. Gli-3is very important for normal limb and brain development and is implicated in thetransduction of Shh signal. Gli-3 is a nuclear protein expressed in a wide variety of normaladult tissues, including lung, colon, spleen, placenta, testis, and myometrium. Defects inGli-3 are the cause of Greig cephalo-poly-syndactyly syndrome (GCPS), an autosomaldominant disorder-affecting limb and cranio-facial development. Two isoforms of humanGli-3 have been reported. One is the full-length protein at ~170-190kDa and the other is atruncated isoform at ~80kDa.

Specificity: This antibody recognises GLI-Kruppel Family Member GLI3. Species Reactivity: Tested: Human. Expected from sequence similarity: Chimpanzee, Chicken, Canine (Dog), Frog, Monkey,Mouse, Quail and Rat.

Application Details

Application Notes: ELISA (1/6000-1/30000). Western Blot (1/500-1/2000). Immunohistochemistry on Paraffin Sections (1.25 µg/ml). This antibody was validated for use in immunohistochemistry on a panel of 21formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigenretrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides wereincubated with biotinylated secondary antibody LS-D1, followed by alkalinephosphatase-streptavidin and chromogen. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.84 mg/ml

Purification: Aff - Purified

Buffer: 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 with 0.01%(w/v) Sodium Azide as preservative.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Dilute only prior to immediate use. Avoid cycles of freezing and thawing. Shelf life: One year from despatch.

Restrictions: For Research Use only