ANO1 antibody (C-Term)

Catalog No. ABIN3042546

Product Details anti-ANO1 Antibody

Target: ANO1 (Anoctamin 1, Calcium Activated Chloride Channel (ANO1))

Binding Specificity: AA 927-943, C-Term

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ANO1 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Anoctamin-1(ANO1) detection. Tested with WB in Human,Mouse,Rat.

Sequence: KVLMVELFMR EEQDKQQ

Cross-Reactivity (Details): Predicted Cross Reactivity: human, mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Anoctamin-1(ANO1) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: anoctamin 1, calcium activated chloride channel
Protein Name: Anoctamin-1

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human TMEM16A(927-943aa KVLMVELFMREEQDKQQ), different from the related mouse and rat sequences by one amino acid.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Rat, Predicted Species: Human, Mouse
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for ANO1

Target: ANO1 (Anoctamin 1, Calcium Activated Chloride Channel (ANO1))

Alternative Name: ANO1 (ANO1 Products)

Synonyms: DOG1 antibody, ORAOV2 antibody, TAOS2 antibody, TMEM16A antibody, Tmem16a antibody, anoctamin 1 antibody, anoctamin 1, calcium activated chloride channel antibody, Ano1 antibody, ANO1 antibody

Background: Anoctamin-1(ANO1), also known as oral cancer overexpressed 2(ORAOV2) or tumor-amplified and overexpressed sequence 2(TMEM16A), is a protein that in humans is encoded by the ANO1 gene. This gene belongs to a family of membrane proteins containing 8 transmembrane segments, and it is mapped to 11q13.3. ANO1 is a candidate calcium-activated chloride channel that mediates receptor-activated chloride currents in diverse physiologic processes, and it is though to be responsible for a voltage-sensitive calcium-activated chloride current. Its overexpression was reported in esophageal squamous cell carcinoma and breast cancer progression Crofelemer, an antidiarrhoeal, inhibits this channel. ANO1 has eight transmembrane domains, its pore is large and non-selective, allowing other negatively charged species to permeate.

Synonyms: ANO 1 antibody|ANO1 antibody|ANO1_HUMAN antibody|Anoctamin 1 antibody|Anoctamin 1 calcium activated chloride channel antibody|Anoctamin-1 antibody|Anoctamin1 antibody|Discovered on gastrointestinal stromal tumors protein 1 antibody|DOG 1 antibody|FLJ10261 antibody|Membrane protein antibody|Oral cancer overexpressed 2 antibody|Oral cancer overexpressed protein 2 antibody|ORAOV 2 antibody|ORAOV2 antibody|TAOS 2 antibody|TAOS2 antibody|TMEM 16A antibody|TMEM16A antibody|Transmembrane protein 16A(eight membrane spanning domains) antibody|Transmembrane protein 16A antibody|Tumor amplified and overexpressed sequence 2 antibody|Tumor-amplified and overexpressed sequence 2 antibody