CNN1 antibody (C-Term)

Catalog No. ABIN3042824

Product Details anti-CNN1 Antibody

Target: CNN1 (Calponin 1 (CNN1))

Binding Specificity: AA 283-297, C-Term

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CNN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Calponin-1(CNN1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequence: EPAHNHHAHN YYNSA

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Calponin-1(CNN1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: calponin 1, basic, smooth muscle
Protein Name: Calponin-1

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Calponin(283-297aa EPAHNHHAHNYYNSA), different from the related mouse and rat sequences by two amino acids.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

References for anti-CNN1 antibody (ABIN3042824)

Fan, Han, Feng, Fang, Zhang, Hu, Hao, Qi, Zhang, Li: "Functional and Proteomic Investigations Reveal Major Royal Jelly Protein 1 Associated with Anti-hypertension Activity in Mouse Vascular Smooth Muscle Cells." in: Scientific reports, Vol. 6, pp. 30230, (2018) (PubMed).

Qu, Hui, Wang, Tang, Zhong, Liu, Li, Feng, He et al.: "Reduced Expression of the Extracellular Calcium-Sensing Receptor (CaSR) Is Associated with Activation of the Renin-Angiotensin System (RAS) to Promote Vascular Remodeling in the Pathogenesis of ..." in: PLoS ONE, Vol. 11, Issue 7, pp. e0157456, (2016) (PubMed).

Yuan, Liu, Li, Li, Sun, Xu, Man, Fu: "Effects of BMSCs interactions with adventitial fibroblasts in transdifferentiation and ultrastructure processes." in: International journal of clinical and experimental pathology, Vol. 7, Issue 7, pp. 3957-65, (2015) (PubMed).

Liu, Chen, Zeng, Cui, Ning, Wang, Belguise, Wang, Qian, Lu, Yi: "Bone morphogenic protein-2 regulates the myogenic differentiation of PMVECs in CBDL rat serum-induced pulmonary microvascular remodeling." in: Experimental cell research, Vol. 336, Issue 1, pp. 109-18, (2015) (PubMed).

Target Details for CNN1

Target: CNN1 (Calponin 1 (CNN1))

Alternative Name: CNN1 (CNN1 Products)

Synonyms: CN antibody, CnnI antibody, SMCC antibody, Sm-Calp antibody, XclpH3 antibody, clpH3 antibody, cnn2 antibody, calponin 1 antibody, calponin 1, basic, smooth muscle L homeolog antibody, cnn1 antibody, Cnn1 antibody, CNN1 antibody, cnn1.L antibody

Background: Calponin is a basic 34-kD protein first isolated from chicken gizzard and bovine aorta. This gene is mapped to 19p13.2. It is specifically expressed in smooth muscle and binds calmodulin, actin, and tropomyosin. It is able to inhibit the ATPase activity of myosin and is though to play a role in smooth muscle contraction. Calponin is a smooth muscle specific, actin-, tropomyosin- and calmodulin-binding protein though to be involved in some way in the regulation or modulation of contraction.

Synonyms: Basic calponin antibody|Calponin 1 antibody|Calponin 1 basic smooth muscle antibody|Calponin H1 antibody|Calponin H1 smooth muscle antibody|Calponin-1 antibody|Calponin1 antibody|Calponins basic antibody|CNN 1 antibody|Cnn1 antibody|CNN1_HUMAN antibody|Sm Calp antibody|SMCC antibody|smooth muscle antibody

UniProt: P51911