CD63 antibody (AA 97-238)

Catalog No. ABIN3043395

Product Details anti-CD63 Antibody

Target: CD63

Binding Specificity: AA 97-238

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD63 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for CD63 antigen(CD63) detection. Tested with WB, IHC-P in Human.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for CD63 antigen(CD63) detection. Tested with WB, IHC-P in Human.
Gene Name: CD63 Molecule
Protein Name: CD63 antigen

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CD63 recombinant protein (Position: E97-M238). Human CD63 shares 74% and 73% amino acid (aa) sequence identity with mouse and rat CD63, respectively.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for CD63 is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-CD63 antibody (ABIN3043395)

Ren, Wang, Yuan, Wang: "Dendritic cells loaded with HeLa-derived exosomes simulate an antitumor immune response." in: Oncology letters, Vol. 15, Issue 5, pp. 6636-6640, (2018) (PubMed).

Du, Dong, Zhao, Fu, Wang, Chen, Ou, Li, Sun, Tang, Song: "Immunostimulatory and anti-neoplasm effects of a novel palindrome CpG oligodeoxynucleotide in mice." in: Acta pharmacologica Sinica, Vol. 33, Issue 8, pp. 1047-54, (2012) (PubMed).

Target Details for CD63

Target: CD63

Alternative Name: CD63 (CD63 Products)

Synonyms: C75951 antibody, ME491 antibody, Tspan30 antibody, LAMP-3 antibody, MLA1 antibody, OMA81H antibody, TSPAN30 antibody, TSP29FA antibody, CD63 antigen antibody, CD63 molecule antibody, Cd63 molecule antibody, Cd63 antibody, CD63 antibody

Background: CD63 antigen is a protein that in humans is encoded by the CD63 gene. The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. It is mapped to 12q13.2. CD63 is mainly associated with membranes of intracellular vesicles, although cell surface expression may be induced. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression.

Synonyms: Lysosomal associated membrane protein 3 antibody|CD 63 antibody|CD63 antibody|CD63 antigen (melanoma 1 antigen) antibody|CD63 antigen antibody|CD63 antigen melanoma 1 antigen antibody|CD63 Molecule antibody|CD63_HUMAN antibody|gp55 antibody|granulophysin antibody|LAMP 3 antibody|LAMP-3 antibody|LAMP3 antibody|LIMP antibody|Lysosomal-associated membrane protein 3 antibody|lysosome associated membrane glycoprotein 3 antibody|Mast cell antigen AD1 antibody|ME491 antibody|melanoma 1 antigen antibody|Melanoma associated antigen ME491 antibody|Melanoma associated antigen MLA1 antibody|Melanoma-associated antigen ME491 antibody|MGC72893 antibody|MLA 1 antibody|MLA1 antibody|NGA antibody|ocular melanoma associated antigen antibody|Ocular melanoma-associated antigen antibody|OMA81H antibody|PTLGP40 antibody|Tetraspanin 30 antibody|Tetraspanin-30 antibody|Tspan 30 antibody|Tspan-30 antibody|TSPAN30 antibody

Gene ID: 967

UniProt: P08962