NK2 Homeobox 5 antibody (Middle Region)

Catalog No. ABIN3043568

Product Details anti-NK2 Homeobox 5 Antibody

Target: NK2 Homeobox 5 (NKX2-5)

Binding Specificity: AA 103-132, Middle Region

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NK2 Homeobox 5 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Homeobox protein Nkx-2.5(NKX2-5) detection. Tested with WB in Human,Mouse.

Sequence: AKDPRAEKKE LCALQKAVEL EKTEADNAER

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Homeobox protein Nkx-2.5(NKX2-5) detection. Tested with WB in Human,Mouse.
Gene Name: NK2 homeobox 5
Protein Name: Homeobox protein Nkx-2.5

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Nkx2.5 (103-132aa AKDPRAEKKELCALQKAVELEKTEADNAER), different from the related mouse and rat sequences by five amino acids.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, The detection limit for Nkx2.5 is approximately 0.1 ng/lane under reducing conditions.
Notes: Tested Species: Species with positive results.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for NK2 Homeobox 5

Target: NK2 Homeobox 5 (NKX2-5)

Alternative Name: NKX2-5 (NKX2-5 Products)

Synonyms: CHNG5 antibody, CSX antibody, CSX1 antibody, HLHS2 antibody, NKX2.5 antibody, NKX2E antibody, NKX4-1 antibody, VSD3 antibody, Csx antibody, Nkx-2.5 antibody, Nkx2.5 antibody, tinman antibody, nk2.5 antibody, nkx2-5 antibody, zgc:111912 antibody, AR2 antibody, XNkx2-5 antibody, csx antibody, nkx-2.5 antibody, nkx2-5b antibody, nkx2.5 antibody, NKX2-5 antibody, Nkx2-5 antibody, csx1 antibody, nkx2e antibody, XNkx-2.5 antibody, CNKX-2.5 antibody, NKX-2.5 antibody, NK2 homeobox 5 antibody, NK2 homeobox 5 S homeolog antibody, NKX2-5 antibody, Nkx2-5 antibody, nkx2.5 antibody, nkx2-5.S antibody, nkx2-5 antibody

Background: Homeobox protein Nkx-2.5, also known as NKX2E or CSX is a protein that in humans is encoded by the NKX2-5 gene. It is mapped to 5q35.1. Homeobox-containing genes play critical roles in regulating tissue-specific gene expression essential for tissue differentiation, as well as determining the temporal and spatial patterns of development. Nkx2.5 and Tbx5 directly bound to the promoter of the gene encoding cardiac-specific natriuretic peptide precursor type A (NPPA) in tandem, and both transcription factors showed synergistic activation. The cardiac homeobox protein Nkx2.5 is essential in cardiac development, and mutations in CSX (which encodes Nkx2.5) cause various congenital heart malformations.

Synonyms: Cardiac-specific homeobox 1 antibody|Cardiac-specific homeobox antibody|CHNG5 antibody|CSX antibody|CSX1 antibody|FLJ52202 antibody|FLJ97166 antibody|FLJ97195 antibody|FLJ97197 antibody|FLJ99536 antibody|HLHS2 antibody|Homeobox protein CSX antibody|Homeobox protein NK-2 homolog E antibody|Homeobox protein Nkx 2.5 antibody|NK2 homeobox 5 antibody|NK2 transcription factor related locus 5 antibody|NK2 transcription factor related, locus 5 (Drosophila) antibody|Nkx2-5 antibody|NKX2.5 antibody|Nkx2E antibody|NKX4-1 antibody|Tinman paralog antibody|VSD3 antibody

Gene ID: 1482

UniProt: P52952

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development