GAP43 antibody (C-Term)

Catalog No. ABIN3043685

Product Details anti-GAP43 Antibody

Target: GAP43 (Growth Associated Protein 43 (GAP43))

Binding Specificity: AA 216-238, C-Term

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GAP43 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Neuromodulin(GAP43) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequence: KPKESARQDE GKEEEPEADQ EHA

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Neuromodulin(GAP43) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: growth associated protein 43
Protein Name: Neuromodulin

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human GAP43(216-238aa KPKESARQDEGKEEEPEADQEHA), different from the related mouse and rat sequences by one amino acid.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-GAP43 antibody (ABIN3043685)

Liu, Lv, Zhu, Wu, Zhang, Xu, Zheng, Zhao: "Salidroside promotes peripheral nerve regeneration based on tissue engineering strategy using Schwann cells and PLGA: in vitro and in vivo." in: Scientific reports, Vol. 7, pp. 39869, (2018) (PubMed).

Tan, Yu, Song, Zou, Xu, Liu: "Expression of MicroRNA-29a Regulated by Yes-Associated Protein Modulates the Neurite Outgrowth in N2a Cells." in: BioMed research international, Vol. 2017, pp. 5251236, (2018) (PubMed).

Liu, Liu, Zeng et al.: "Chronic Unpredictable Mild Stress Causing Cardiac and Thoracic Spinal Cord Electrophysiological Abnormalities May Be Associated with Increased Cardiac Expression of Serotonin and Growth-Associated ..." in: BioMed research international, Vol. 2018, pp. 8697913, (2018) (PubMed).

Yuan, Sun, Yu, An: "Human microvascular endothelial cell promotes the development of dorsal root ganglion neurons via BDNF pathway in a co-culture system." in: Bioscience, biotechnology, and biochemistry, Vol. 81, Issue 7, pp. 1335-1342, (2017) (PubMed).

Zhu, Liu, Wang, Nie, He, Zhang, Liu, Su: "Lentiviral-mediated growth-associated protein-43 modification of bone marrow mesenchymal stem cells improves traumatic optic neuropathy in rats." in: Molecular medicine reports, Vol. 12, Issue 4, pp. 5691-700, (2016) (PubMed).

Zou, Ding, Shi, Xu, Gong, Zhang, Liu: "MicroRNA-29c/PTEN pathway is involved in mice brain development and modulates neurite outgrowth in PC12 cells." in: Cellular and molecular neurobiology, Vol. 35, Issue 3, pp. 313-22, (2015) (PubMed).

Zou, Ding, Wang, Xiong, Peng, Du, Zhang, Liu, Gong: "MicroRNA-29A/PTEN pathway modulates neurite outgrowth in PC12 cells." in: Neuroscience, Vol. 291, pp. 289-300, (2015) (PubMed).

Wang, Liu, Jin, Xu, Wang, Han, Yin: "Fructus Corni extract-induced neuritogenesis in PC12 cells is associated with the suppression of stromal interaction molecule 1 expression and inhibition of Ca2+ influx." in: Experimental and therapeutic medicine, Vol. 9, Issue 5, pp. 1773-1779, (2015) (PubMed).

Chen, Wei, Nie, Lin, Tian, Liu, Yu, Cheng, Yan, Wang, Liu, Deng, Lai, Zhou, Zhang, Lin, Chen: "?-Asarone prevents autophagy and synaptic loss by reducing ROCK expression in asenescence-accelerated prone 8 mice." in: Brain research, Vol. 1552, pp. 41-54, (2014) (PubMed).

Nie, Deng, Yang, Liu, Zhang, Wen: "Axonal regeneration and remyelination evaluation of chitosan/gelatin-based nerve guide combined with transforming growth factor-?1 and Schwann cells." in: Cell biochemistry and biophysics, Vol. 68, Issue 1, pp. 163-72, (2014) (PubMed).

Target Details for GAP43

Target: GAP43 (Growth Associated Protein 43 (GAP43))

Alternative Name: GAP43 (GAP43 Products)

Synonyms: B-50 antibody, PP46 antibody, Basp2 antibody, GAP-43 antibody, b-50 antibody, gap-43 antibody, gap43b antibody, pp46 antibody, cb310 antibody, gap antibody, gap43 antibody, gap43a antibody, growth associated protein 43 antibody, growth associated protein 43 L homeolog antibody, growth associated protein 43 S homeolog antibody, GAP43 antibody, Gap43 antibody, gap43.L antibody, gap43 antibody, gap43.S antibody

Background: Growth Associated Protein-43(GAP-43), also known as nerve growth-related growth peptide GAP43, shares a high degree of homology between the sequence of the human gene and the rat gene. GAP-43 is considered a crucial component of an effective regenerative response in the nervous system. Somatic cell hybrids demonstrate localization of the GAP-43 gene to human chromosome 3 and to mouse chromosome 16. GAP-43 has been termed a "growth" or "plasticity" protein because it is expressed at high levels in neuronal growth cones during development and during axonal regeneration. GAP-43 regulates growth of axons and modulates the formation of new connections.

Synonyms: Axonal membrane protein GAP 43 antibody|Axonal membrane protein GAP-43 antibody|B 50 antibody|Calmodulin binding protein P 57 antibody|F1 antibody|GAP 43 antibody|GAP43 antibody|Growth Associated Protein 43 antibody|Growth-associated protein 43 antibody|Nerve Growth Related Peptide antibody|Nerve growth related peptide GAP43 antibody|NEUM_HUMAN antibody|Neural phosphoprotein B 50 antibody|Neural phosphoprotein B-50 antibody|Neuromodulin antibody|Neuron growth associated protein 43 antibody|pp46 antibody|Protein F1 antibody|QtrA-11580 antibody|QtrA-13071 antibody

UniProt: P17677