XAF1 antibody (C-Term)

Catalog No. ABIN3043705

Product Details anti-XAF1 Antibody

Target: XAF1 (XiAP Associated Factor 1 (XAF1))

Binding Specificity: AA 283-301, C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This XAF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for XIAP-associated factor 1(XAF1) detection. Tested with WB, IHC-P in Human.

Sequence: QEKCRWLASS KGKQVRNFS

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for XIAP-associated factor 1(XAF1) detection. Tested with WB, IHC-P in Human.
Gene Name: XIAP associated factor 1
Protein Name: XIAP-associated factor 1

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human XAF1(283-301aa QEKCRWLASSKGKQVRNFS).

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for XAF1

Target: XAF1 (XiAP Associated Factor 1 (XAF1))

Alternative Name: XAF1 (XAF1 Products)

Synonyms: BIRC4BP antibody, HSXIAPAF1 antibody, XIAPAF1 antibody, Fbxo39 antibody, XIAP associated factor 1 antibody, xaf1 antibody, XAF1 antibody, Xaf1 antibody

Background: XIAP associated factor-1, also known as XAF1, is a human gene. X-linked inhibitor of apoptosis(XIAP, MIM 300079) is a potent member of the IAP family. All members of this family possess baculoviral IAP(BIR) repeats, cysteine-rich domains of approximately 80 amino acids that bind and inhibit caspases. XAF1 antagonizes the anticaspase activity of XIAP and may be important in mediating apoptosis resistance in cancer cells. And alteration in XAF1 and XIAP RNA expression levels may lead to increased apoptotic resistance and proliferation due to unregulated XIAP function in cancer cells.

Synonyms: BIRC 4 binding protein antibody|BIRC4 binding protein antibody|BIRC4-binding protein antibody|BIRC4BP antibody|HSXIAPAF 1 antibody|HSXIAPAF1 antibody|XAF 1 antibody|XAF1 antibody|XAF1_HUMAN antibody|XIAP associated factor 1 antibody|XIAP-associated factor 1 antibody|XIAPAF1 antibody