ATP5H antibody (AA 2-161)

Catalog No. ABIN3043795

Product Details anti-ATP5H Antibody

Target: ATP5H (ATP Synthase, H+ Transporting, Mitochondrial Fo Complex, Subunit D (ATP5H))

Binding Specificity: AA 2-161

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP5H antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Purpose: Rabbit IgG polyclonal antibody for ATP synthase subunit d, mitochondrial(ATP5H) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for ATP synthase subunit d, mitochondrial(ATP5H) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Gene Name: ATP synthase, H+ transporting, mitochondrial Fo complex, subunit d
Protein Name: ATP synthase subunit d, mitochondrial

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ATP5H recombinant protein (Position: A2-L161). Human ATP5H shares 81% and 78% amino acid (aa) sequence identity with mouse and rat ATP5H, respectively.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat, The detection limit for ATP5H is approximately 0.1 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for ATP5H

Target: ATP5H (ATP Synthase, H+ Transporting, Mitochondrial Fo Complex, Subunit D (ATP5H))

Alternative Name: ATP5H (ATP5H Products)

Synonyms: 0610009D10Rik antibody, ATPQ antibody, Atp5jd antibody, Atpq antibody, ATP synthase, H+ transporting, mitochondrial Fo complex subunit D L homeolog antibody, ATP synthase, H+ transporting, mitochondrial Fo complex subunit D antibody, ATP synthase, H+ transporting, mitochondrial F0 complex, subunit D antibody, ATP synthase, H+ transporting, mitochondrial Fo complex, subunit d antibody, atp5h.L antibody, atp5h antibody, ATP5H antibody, Atp5h antibody

Background: ATP5H is also known as ATPQ. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the d subunit of the Fo complex. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. In addition, three pseudogenes are located on chromosomes 9, 12 and 15.

Synonyms: ATP synthase D chain mitochondrial antibody|ATP synthase H+ transporting mitochondrial F1F0 subunit antibody|ATP synthase H+ transporting mitochondrial F1F0 subunit d antibody|ATP synthase subunit d antibody|ATP synthase subunit d, mitochondrial antibody|ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d antibody|ATP5H antibody|ATP5H_HUMAN antibody|ATP5JD antibody|ATPase subunit d antibody|ATPQ antibody|mitochondrial antibody|My032 protein antibody

Gene ID: 10476

UniProt: O75947

Pathways: Proton Transport, Ribonucleoside Biosynthetic Process