SNRPN antibody (N-Term)

Catalog No. ABIN3043934

Product Details anti-SNRPN Antibody

Target: SNRPN (Small Nuclear Ribonucleoprotein Polypeptide N (SNRPN))

Binding Specificity: AA 11-39, N-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SNRPN antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Small nuclear ribonucleoprotein-associated protein N(SNRPN) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequence: QHIDYRMRCI LQDGRIFIGT FKAFDKHMN

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Small nuclear ribonucleoprotein-associated protein N(SNRPN) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: small nuclear ribonucleoprotein polypeptide N
Protein Name: Small nuclear ribonucleoprotein-associated protein N

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human SNRPN (11-39aa QHIDYRMRCILQDGRIFIGTFKAFDKHMN), identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat, The detection limit for SNRPN is approximately 0.1 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for SNRPN

Target: SNRPN (Small Nuclear Ribonucleoprotein Polypeptide N (SNRPN))

Alternative Name: SNRPN (SNRPN Products)

Synonyms: SNRB' antibody, SNRPB' antibody, Snrpn antibody, zgc:77315 antibody, HCERN3 antibody, PWCR antibody, RT-LI antibody, SM-D antibody, SMN antibody, SNRNP-N antibody, SNURF-SNRPN antibody, sm-N antibody, 2410045I01Rik antibody, Peg4 antibody, Pwcr1 antibody, FE 294 antibody, FE 294 psi antibody, FE294 antibody, FE294 psi antibody, SNURF antibody, SNRPN antibody, QflA-11237 antibody, QtrA-10022 antibody, Sm-N antibody, SmN antibody, snRNP-N antibody, small nuclear ribonucleoprotein polypeptide-like antibody, small nuclear ribonucleoprotein polypeptides B and B1 antibody, small nuclear ribonucleoprotein polypeptide N antibody, small nuclear ribonucleoprotein N antibody, small nuclear ribonucleoparticle-associated protein (snRNP) mRNA, clone Sm51 antibody, SNRPN upstream reading frame antibody, Small nuclear ribonucleoprotein-associated protein N antibody, SNRPL antibody, snrpb antibody, SNRPN antibody, Snrpn antibody, snrpn antibody, SNURF antibody

Background: SNRPN(Small Nuclear Ribonucleoprotein Polypeptide N), also called SMN, is a bicistronic imprinted gene that encodes 2 polypeptides, the SmN splicing factor, which is involved in RNA processing, and the SNRPN upstream reading frame (SNURF) polypeptide. The protein encoded by this gene is one polypeptide of a small nuclear ribonucleoprotein complex and belongs to the snRNP SMB/SMN family. SNRPN also encodes a long alternatively spliced transcript containing several small nucleolar RNAs (snoRNAs) and extends downstream to partially overlap the UBE3A gene in the antisense orientation. PWS arises from loss of function of genes in this region expressed exclusively from the paternal chromosome, suggesting that SNRPN may play a role in its etiology. The SNRPN gene is mapped on 15q11.2. Analysis of maternal DNA and of SNRPN cDNA confirmed that the maternal allele is not expressed in fetal brain and heart. Deletions in the transcription unit of the imprinted SNRPN gene occur in patients who have PWS or Angelman syndrome because of a parental imprint switch failure in this chromosomal domain.

Synonyms: HCERN3 antibody|PWCR antibody|RSMN_HUMAN antibody|RT LI antibody|RTLI antibody|SM D antibody|Sm N antibody|Sm protein D antibody|Sm protein N antibody|Sm-D antibody|Sm-N antibody|Small nuclear ribonucleoprotein associated protein N antibody|Small nuclear ribonucleoprotein polypeptide N antibody|Small nuclear ribonucleoprotein-associated protein N antibody|SMD antibody|SmN antibody|SNRNP N antibody|snRNP-N antibody|SNRNPN antibody| SNRPN antibody|SNURF SNRPN antibody|Tissue specific splicing protein antibody|Tissue-specific-splicing protein antibody

Gene ID: 6638

UniProt: P63162