TRAF4 antibody (C-Term)

Catalog No. ABIN3044048

Product Details anti-TRAF4 Antibody

Target: TRAF4 (TNF Receptor-Associated Factor 4 (TRAF4))

Binding Specificity: AA 437-454, C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRAF4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for TNF receptor-associated factor 4(TRAF4) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequence: PKFISHQDIR KRNYVRDD

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for TNF receptor-associated factor 4(TRAF4) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: TNF receptor-associated factor 4
Protein Name: TNF receptor-associated factor 4

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human TRAF4(437-454aa PKFISHQDIRKRNYVRDD), identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat, The detection limit for TRAF4 is approximately 0.5 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for TRAF4

Target: TRAF4 (TNF Receptor-Associated Factor 4 (TRAF4))

Alternative Name: TRAF4 (TRAF4 Products)

Synonyms: CART1 antibody, MLN62 antibody, RNF83 antibody, A530032M13Rik antibody, msp2 antibody, traf4 antibody, cart1 antibody, mln62 antibody, rnf83 antibody, traf-4 antibody, traf4l antibody, zgc:77418 antibody, TNF receptor associated factor 4 antibody, Tnf receptor associated factor 4 antibody, tnf receptor-associated factor 4b antibody, TNF receptor associated factor 4 L homeolog antibody, tnf receptor-associated factor 4a antibody, TRAF4 antibody, Traf4 antibody, traf4b antibody, traf4.L antibody, traf4a antibody

Background: TRAF4(TNF Receptor-Associated Factor 4), also called CART1 or MLN62, is a protein that in humans is encoded by the TRAF3 gene. TRAF4 is a member of the TNF receptor associated factor(TRAF) family, a family of scaffold proteins. Tomasetto et al.(1995) identified genes that are overexpressed in breast cancer by differential screening of cDNAs from breast cancer-derived metastatic axillary lymph nodes. The TRAF4 cDNA, which they designated MLN62, was mapped to 17q11-q12 by radioisotopic in situ hybridization. To investigate the biologic function of TRAF4, Regnier et al.(2002) generated Traf4-deficient mice by gene disruption.

Synonyms: CART 1 antibody|CART1 antibody|cysteine rich domain associated with ring and TRAF domain antibody|Cysteine rich domain associated with RING and Traf domains protein 1 antibody|Cysteine-rich domain associated with RING and Traf domains protein 1 antibody|Malignant 62 antibody|metastatic lymph node gene 62 protein antibody|MLN 62 antibody|MLN62 antibody|RING finger protein 83 antibody|RNF 83 antibody|RNF83 antibody|TNF receptor associated factor 4 antibody|TNF receptor-associated factor 4 antibody|TRAF 4 antibody|Traf4 antibody|TRAF4_HUMAN antibody|tumor necrosis receptor-associated factor 4A antibody