TRAM2 antibody (C-Term)

Catalog No. ABIN3044050

Product Details anti-TRAM2 Antibody

Target: TRAM2 (Translocation Associated Membrane Protein 2 (TRAM2))

Binding Specificity: AA 309-325, C-Term

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRAM2 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Translocating chain-associated membrane protein 2(TRAM2) detection. Tested with WB in Human,Mouse,Rat.

Sequence: RFIHSQLRHW REYWKEQ

Cross-Reactivity (Details): Predicted Cross Reactivity: human, rat
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Translocating chain-associated membrane protein 2(TRAM2) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: translocation associated membrane protein 2
Protein Name: Translocating chain-associated membrane protein 2

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of mouse TRAM2(309-325aa RFIHSQLRHWREYWKEQ), identical to the related rat sequence, and different from the related human sequence by one amino acid.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse, Predicted Species: Human, Rat, The detection limit for TRAM2 is approximately 5 ng/lane under reducing conditions.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for TRAM2

Target: TRAM2 (Translocation Associated Membrane Protein 2 (TRAM2))

Alternative Name: TRAM2 (TRAM2 Products)

Synonyms: C330003D03Rik antibody, wu:fb20c11 antibody, wu:fi23a12 antibody, zgc:111902 antibody, zgc:55869 antibody, zgc:76937 antibody, tram2 antibody, translocation associated membrane protein 2 antibody, tram-like protein antibody, TraM-like protein antibody, translocating chain-associating membrane protein 2 antibody, translocation associated membrane protein 2 L homeolog antibody, TRAM2 antibody, CJE0444 antibody, BT_p548215 antibody, traM antibody, CJJ81176_0418 antibody, Tram2 antibody, tram2 antibody, tram2.L antibody

Background: TRAM2(Translocation-Associating Membrane Protein 2), also called KIAA0057, is a protein that in humans is encoded by the TRAM2 gene. TRAM2 is a component of the translocon, a gated macromolecular channel that controls the posttranslational processing of nascent secretory and membrane proteins at the endoplasmic reticulum(ER) membrane. By genomic sequence analysis, Onuchic et al.(1999) mapped the TRAM2 gene to chromosome 6p21.1-p12 near the PKHD1 gene. Upon activation, quiescent hepatic stellate cells proliferate, change morphologically into myofibroblasts, and increase their synthesis of extracellular matrix proteins. Stefanovic et al.(2004) demonstrated that both TRAM2 and collagen type I are upregulated in activated rat and human hepatic stellate cells.

Synonyms: C330003D03Rik antibody|KIAA0057 antibody|MGC25725 antibody|OTTHUMP00000016607 antibody|TRAM like protein antibody|Tram2 antibody|TRAM2_HUMAN antibody|Translocating chain associating membrane protein 2 antibody|Translocating chain-associated membrane protein 2 antibody|Translocation associated membrane protein 2 antibody

UniProt: Q924Z5