FSTL3 antibody (Middle Region)

Catalog No. ABIN3044124

Product Details anti-FSTL3 Antibody

Target: FSTL3 (Follistatin-Like 3 (Secreted Glycoprotein) (FSTL3))

Binding Specificity: AA 145-159, Middle Region

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FSTL3 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Follistatin-related protein 3(FSTL3) detection. Tested with WB in Human,Mouse,Rat.

Sequence: ECELRAARCR GHPDL

Cross-Reactivity (Details): Predicted Cross Reactivity: mouse, rat
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Follistatin-related protein 3(FSTL3) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: follistatin-like 3(secreted glycoprotein)
Protein Name: Follistatin-related protein 3

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human FSTL3(145-159aa ECELRAARCRGHPDL), different from the related rat and mouse sequences by one amino acid.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat, The detection limit for FSTL3 is approximately 5 ng/lane under reducing conditions.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for FSTL3

Target: FSTL3 (Follistatin-Like 3 (Secreted Glycoprotein) (FSTL3))

Alternative Name: FSTL3 (FSTL3 Products)

Synonyms: E030038F23Rik antibody, Flrg antibody, FLRG antibody, FSRP antibody, follistatin-like 3 antibody, follistatin like 3 antibody, Fstl3 antibody, FSTL3 antibody

Background: FSTL3(Follistatin-Like 3) also known as FLRG or FOLLISTATIN-RELATED GENE, is a member of the follistatin-module protein family, which is composed of extracellular matrix-associated glycoproteins though to act in a paracrine manner to bind morphogens or growth/differentiation factors and regulate their activity during development. The FSTL3 gene extends over 7 kbp and contains 5 exons. By Southern blot analysis of somatic cell hybrids and FISH, Hayette et al.(1998) localized the FSTL3 gene to chromosome 19p13. Using recombinant mouse Fstl3, Tsuchida et al.(2000) found that Fstl3bound both activin and BMP2 and had a higher affinity for activin. Overexpression of Fstl3 inhibited BMP2-induced cell signaling in a reporter assay. In addition to the translocation in a case of B-cell chronic lymphocytic leukemia from which FSTL3 was isolated, Hayette et al.(1998) also observed rearrangement of the FSTL3 gene in a case of B-cell non-Hodgkin lymphoma and in a case of B-cell mantle zone lymphoma, suggesting that FSTL3 may be involved in the leukemogenesis process.

Synonyms: FLRG antibody|follistatin-like 3 antibody|follistatin-like 3 glycoprotein antibody|Follistatin-like protein 3 antibody|Follistatin-related gene protein antibody|Follistatin-related protein 3 antibody|FSRP antibody|Fstl3 antibody|FSTL3_HUMAN antibody

UniProt: O95633