CSTB antibody (Middle Region)

Catalog No. ABIN3044489

Product Details anti-CSTB Antibody

Target: CSTB (Cystatin B (Stefin B) (CSTB))

Binding Specificity: AA 26-45, Middle Region

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CSTB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Cystatin-B(CSTB) detection. Tested with WB, IHC-P in Human.

Sequence: QLEEKENKKF PVFKAVSFKS

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Cystatin-B(CSTB) detection. Tested with WB, IHC-P in Human.
Gene Name: cystatin B(stefin B)
Protein Name: Cystatin-B

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Stefin B(26-45aa QLEEKENKKFPVFKAVSFKS).

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for CSTB is approximately 30 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for CSTB

Target: CSTB (Cystatin B (Stefin B) (CSTB))

Alternative Name: CSTB (CSTB Products)

Synonyms: CST6 antibody, EPM1 antibody, EPM1A antibody, PME antibody, STFB antibody, ULD antibody, CSTB antibody, Cyb antibody, Epm1 antibody, Stfb antibody, MGC189005 antibody, pme antibody, cst6 antibody, epm1 antibody, stfb antibody, AA960480 antibody, ARABIDOPSIS THALIANA PHYTOCYSTATIN 6 antibody, ATCYS6 antibody, ATCYSB antibody, cystatin B antibody, LOC100008600 antibody, LOC100136235 antibody, CPI1 antibody, cystatin B antibody, cystatin B (stefin B) antibody, cystatin 14a, tandem duplicate 2 antibody, cystatin-B antibody, CSTB antibody, Cstb antibody, cstb antibody, cst14a.2 antibody, CYSB antibody, cpi1 antibody, LOC101123265 antibody

Background: Cystatin B(CSTB),also called STFB, is a small protein that is a member of the superfamily of cysteine protease inhibitors. It has been isolated from human spleen and liver and its amino acid sequence has been fully determined. The cystatin B gene is located on 21q22.3. It is widely distributed and is localized mostly intracellularly, but has been found extracellularly. The protein is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. Its role is though to be as a protector against the proteinases leaking from lysosomes. A cystatin B multiprotein complex might have a specific cerebellar function, and that the loss of this function might contribute to the etiopathogenesis of EPM1. Upon differentiation to myotubes, CSTB becomes excluded from the nucleus and lysosomes, suggesting that the subcellular distribution of CSTB is dependent on the differentiation status of the cell.

Synonyms: CHROW21 antibody|CPI B antibody|CPI-B antibody|CST6 antibody|CSTB antibody|Cystatin B antibody|Cystatin-B antibody|CYTB antibody|CYTB_HUMAN antibody|EPM1 antibody|EPM1A antibody|Liver thiol proteinase inhibitor antibody|PME antibody|Stefin-B antibody|STFB antibody|ULD antibody

UniProt: P04080

Pathways: Response to Water Deprivation