S1PR1 antibody (C-Term)

Catalog No. ABIN306730

This Rabbit Polyclonal antibody specifically detects S1PR1 in WB. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for S1PR1 antibody (C-Term) (ABIN306730)

Target: S1PR1 (Sphingosine-1-Phosphate Receptor 1 (S1PR1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This S1PR1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-S1PR1 Antibody

Binding Specificity: C-Term

Purpose: Rabbit anti Human Sphingosine 1 Phosphate Receptor 1 (EDG-1) CT

Cross-Reactivity: Human, Mouse, Rat

Characteristics: EDG-1 belongs to a family of G-protein coupled receptors whose ligands are lysophospholipids. The ligand for EDG-1 is sphingosine-1-phosphate. There are 8 known members of the EDG receptor family and they are implicated in mediating growth related effects such as induction of cellular proliferation, alterations in differentiation and survival and suppression of apoptosis. They also evoke cellular effector functions that are dependent on cytoskeletal responses such as contraction, secretion, adhesion and chemotaxis. EDG receptors are developmentally regulated and differ in tissue distribution. They couple to multiple types of G proteins to signal through ras and MAP kinase, rho, phospholipase C and several protein tyrosine kinases. EDG-1 is expressed in cardiovascular, leukocyte- containing and other tissues.

Purification: Ammonium Sulfate Precipitation

Immunogen: Synthetic peptide derived from the C-terminal of the EDG-1 receptor

Isotype: IgG

Application Details

Application Notes: Detects EDG1 receptors at concentration of 5-10 μg/mL by Western blot using a human EDG1 receptor transfected cell line. Detects an approximately 44 kDa band in RH7777 cells transfected with full length human EDG1. Due to low expression of EDG receptors, we recommend use of Pierce Femto Signal substrate for western blot development. Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details for S1PR1

Target: S1PR1 (Sphingosine-1-Phosphate Receptor 1 (S1PR1))

Alternative Name: Sphingosine 1 Phosphate Receptor 1 (EDG-1)

Background: Endothelial cell differentiation gene 1 C-terminal

UniProt: P21453

Pathways: Signaling Events mediated by VEGFR1 and VEGFR2