6x-HIS Tag antibody
| Antigen |
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| Clonality |
Monoclonal (IPA2C5-1) |
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Host
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Application
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| Catalog no. |
ABIN306800 |
| Quantity |
100 ug (1 mg/ml) |
| Price |
210.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 to 7 Business Days |
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Isotype
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IgG2a (Matching secondary antibodies)
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Clone
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IPA2C5-1
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Description
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6x-His tags are a type of tag for expressed proteins. This tag is made up of 6 histidine residues attached to either the N- or C-terminal of a protein. Proteins expressed with this type of tag are then traditionally purified using a divalent metal ion column. However, using an antibody specific to this 6x-His tag, the protein can be analyzed for by Western blot or immunofluorescence techniques, thus eliminating the need for a protein-specific antibody. 6x-His tags are particularly effective for the purification of protein expressed in E. coli systems. This antibody has been found to be effective in detecting proteins expressed using the following vector systems, pET 28, 30 and 32 as well as Invitrogen's pIZ and is specific for 6x-His tags only. Synonyms: Anti-6x-Histidine Tag Antibody.
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Application Notes
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Detects only 6x-His tag by Western blot. Specific for His tags no longer than 6 histidines.
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Concentration
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1 mg/ml
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Storage
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Product should be stored at -20C. Aliquot to avoid freeze/thaw cycles
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Restrictions
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For Research Use only
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Alternatives for antigen "6x-HIS Tag", type "Antibodies"
Alternatives 
