CERK antibody

Catalog No. ABIN306956

The Rabbit Polyclonal anti-CERK antibody is suitable to detect CERK in samples from Human. It has been validated for ELISA and WB.

Quick Overview for CERK antibody (ABIN306956)

Target: CERK (Ceramide Kinase (CERK))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CERK antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Product Details anti-CERK Antibody

Purpose: Rabbit anti Human Ceramide Kinase

Cross-Reactivity: Human

Characteristics: Sphingolipids, in addition to being structural components of membranes, regulate cell-cell and cell-substrate interactions, proliferation, and differentiation. Members of this diverse group of lipids have emerged as a novel class of signaling molecules that also regulate phagocytosis. The mechanisms by which sphingolipids exert these effects remain incompletely defined. More than a decade ago, it was found that ceramide can be phosphorylated to ceramide 1-phosphate (C1P). Ceramide kinase (CERK) and its phosphorylated product ceramide 1-phosphate (C1P) are central players in inflammation and cancer. The product of CERK activity, ceramide 1-phosphate (C1P), has been reported to have mitogenic effects. C1P is a direct activator of cytosolic phospholipase A2 and is involved in arachidonic acid release. CERK is a mediator of Ca2+-dependent degranulation in mast cells. In both arachidonic acid release and mast cell degranulation, the intracellular elevation of Ca2+ is a central event that acts as a regulatory mechanism of CERK activity. C1P is found in brain synaptic vesicles, and plays a role in regulating the secretion of neurotransmitters. CERK activity exists in HL-60 cells where the C1P is derived from ceramide released from sphingomyelin. The expressed kinase has specific ceramide phosphorylating activity. CERKs exist in a variety of cellular organisms, including plants, nematodes, insects, and vertebrates.

Purification: Ammonium Sulfate Precipitation

Immunogen: Synthetic peptide derived from human ceramide kinase protein

Application Details

Application Notes: Antibody can be used for Western blotting (1-10 μg/mL). Can also be used for ELISA. Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details for CERK

Target: CERK (Ceramide Kinase (CERK))

Alternative Name: Ceramide Kinase

Background: Ceramide kinase, Acylsphingosine kinase, hCERK, Lipid kinase 4, LK4,   CERK, KIAA1646

UniProt: Q8TCT0