Sphingosine Kinase 1 (SPHK1) antibody

Antigen: Sphingosine Kinase 1 (SPHK1)

Synonyms: SPHK, Sk1, Spk1, 1110006G24Rik, SK1, Sphk1, DmelCG1747, CG1747, SPHK1, F18E5.160, F18E5_160, SPHINGOSINE KINASE 1

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN308402

Additional Information

Alternative name: SPHK1

Gene ID: 8877, 20698 (mouse), 170897 (rat)

Immunogen: C-DVDLESEKYRRLGE

Format: Affinity-purified

Description: All names and symbols: SPHK1, sphingosine kinase 1, SPHK, SK1, SKase. Official Symbol: SPHK1. RefSeq number(s): NP_068807.2, NP_892010.1

Specificity: This antibody is expected to recognise both reported isoforms (NP_068807.2, NP_892010.1). Human, Mouse, Rat, Dog, Cow

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx. 40 kDa band observed in Human Peripheral Blood Mononucleocyte lysates (calculated MW of 43.9 kDa according to NP_068807.2). Recommended concentration: 2-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Cancer

Restrictions: For Research Use only

Images

ABIN308402 (2µg/ml) staining of Human Peripheral Blood Mononucleocyte lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Kase, Hattori, Jojima et al.: "Globular adiponectin induces adhesion molecule expression through the sphingosine kinase pathway in vascular endothelial cells." in: Life sciences, Vol. 81, Issue 11, pp. 939-43, 2007 (PubMed).