SPHK1 antibody (N-Term)

Catalog No. ABIN391347

Cited in 2+ publications. The Rabbit Polyclonal anti-SPHK1 antibody (Clone RB15394) (ABIN391347) specifically detects SPHK1 in WB and IHC (p). The antibody is reactive with Human samples.

Quick Overview for SPHK1 antibody (N-Term) (ABIN391347)

Target: SPHK1 (Sphingosine Kinase 1 (SPHK1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPHK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB15394

Product Details anti-SPHK1 Antibody

Binding Specificity: AA 59-89, N-Term

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This SPHK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 59-89 amino acids from the N-terminal region of human SPHK1.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. IHC-P: 1:100. IHC-P: 1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C.

Expiry Date: 6 months

References for anti-SPHK1 antibody (ABIN391347)

Kim, Park, Kim, DAgati, Lee: "Isoflurane activates intestinal sphingosine kinase to protect against renal ischemia-reperfusion-induced liver and intestine injury." in: Anesthesiology, Vol. 114, Issue 2, pp. 363-73, (2011) (PubMed).

Di, Kawamura, Gao, Tang, Berdyshev, Vogel, Zhao, Sharma, Bachmaier, Xu, Malik: "A novel function of sphingosine kinase 1 suppression of JNK activity in preventing inflammation and injury." in: The Journal of biological chemistry, Vol. 285, Issue 21, pp. 15848-57, (2010) (PubMed).

Target Details for SPHK1

Target: SPHK1 (Sphingosine Kinase 1 (SPHK1))

Alternative Name: SPHK1

Background: Sphingosine Kinase (SphK) catalyzes the phosphorylation of the lipid sphingosine, creating the bioactive lipid sphingosine-1-phosphate (S1P). S1P subsequently signals through cell surface G protein-coupled receptors, as well as intracellularly, to modulate cell proliferation, survival, motility and differentiation. SphK is an important signaling enzyme which is activated by diverse agents, including growth factors that signal through receptor tyrosine kinases, agents activating G protein-coupled receptors, and immunoglobulin receptors. Two SphK isotypes, SphK-1 and SphK-2, have been cloned, and both isotypes are ubiquitously expressed. SphK-1 has been shown to mediate cell growth, prevention of apoptosis, and cellular transformation, and is upregulated in a variety of human tumors. In contrast, SphK-2 increases apoptosis, and may be responsible for phosphorylating and activating the immunosuppressive drug FTY720.

NCBI Accession: NP_001136073, NP_001136074, NP_068807, NP_892010

UniProt: Q9NYA1

Pathways: VEGF Signaling