SPHK1 antibody (C-Term)

Catalog No. ABIN652302

The Rabbit Polyclonal anti-SPHK1 antibody (Clone RB15395) (ABIN652302) specifically detects SPHK1 in WB, FACS and IHC (p). The antibody is reactive with Human samples.

Quick Overview for SPHK1 antibody (C-Term) (ABIN652302)

Target: SPHK1 (Sphingosine Kinase 1 (SPHK1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPHK1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB15395

Product Details anti-SPHK1 Antibody

Binding Specificity: AA 313-343, C-Term

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This SPHK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 313-343 amino acids from the C-terminal region of human SPHK1.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. WB: 1:1000. IHC-P: 1:50~100. FC: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

Target Details for SPHK1

Target: SPHK1 (Sphingosine Kinase 1 (SPHK1))

Alternative Name: SPHK1

Background: SPHK1 is a novel lipid messenger with both intracellular and extracellular functions. Intracellularly, it regulates proliferation and survival, and extracellularly, it is a ligand for EDG1 (MIM 601974). Various stimuli increase cellular levels of SPP by activation of sphingosine kinase (SPHK), the enzyme that catalyzes the phosphorylation of sphingosine. Competitive inhibitors of SPHK block formation of SPP and selectively inhibit cellular proliferation induced by a variety of factors, including platelet-derived growth factor (e.g., MIM 173430) and serum.

Gene ID: 8877

NCBI Accession: NP_001136073, NP_001136074, NP_068807, NP_892010

UniProt: Q9NYA1

Pathways: VEGF Signaling