Cytokeratin 13 (KRT13) antibody

Antigen: Cytokeratin 13 (KRT13)

Synonyms: K13, CK13, MGC3781, MGC161462, Krt1-13, Krt-1.13

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN308470

Additional Information

Alternative name: KRT13

Gene ID: 3860

Immunogen: C-TSNASGRRTSDVRRP

Format: Affinity-purified

Description: All names and symbols: KRT13, keratin 13, CK13, K13, MGC161462, MGC3781, cytokeratin 13, keratin, type I cytoskeletal 13. Official Symbol: KRT13. RefSeq number(s): NP_705694.2

Specificity: This antibody is expected to recognize the reported isoform a (NP_002265.2) only. Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: Approx. 55kDa band observed in Human Lung, Skeletal Muscle and Skin lysates (calculated MW of 49.6kDa according to NP_705694.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Sajjan, Microbiology. 2003 Apr,149(Pt 4):961-71., PMID: 12686638). Recommended concentration: 0.1-0.3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN308470 (0.1µg/ml) staining of Human Lung lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Matta, Mazzacurati, Schamus et al.: "Kaposi's sarcoma-associated herpesvirus (KSHV) oncoprotein K13 bypasses TRAFs and directly interacts with the IkappaB kinase complex to selectively activate NF-kappaB without JNK activation." in: The Journal of biological chemistry, Vol. 282, Issue 34, pp. 24858-65, 2007 (PubMed).