Checkpoint Kinase 2 (CHEK2) (N-Term) antibody

Details for Product No. ABIN309588
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Antigen
Synonyms CHK-2, CDS1, CHK2, HuCds1, LFS2, PP1425, RAD53, hCds1, Cds1, HUCDS1, Rad53, Chk2, fa66f08, wu:fa66f08, zgc:55865
Epitope
N-Term
(36), (36), (29), (28), (16), (13), (13), (11), (9), (7), (6), (6), (5), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(332), (147), (146), (55), (37), (36), (12), (7), (6), (1)
Host
Rabbit
(274), (65), (1), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(7), (7), (6), (6), (6), (6), (6), (6), (6), (6), (6), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(257), (109), (88), (60), (60), (42), (31), (28), (9), (4), (1), (1)
Pubmed 3 references available
Quantity 50 µg
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Catalog No. ABIN309588
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Immunogen Synthetic peptide directed towards the N terminal of human CHEK2
Sequence MSRESDVEAQ QSHGSSACSQ PHGSVTQSQG SSSQSQGISS SSTSTMPNSS
Predicted Reactivity Human
Characteristics This is a rabbit polyclonal antibody against CHEK2. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name CHEK2
Background In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. CHEK2 is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, CHEK2 is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in its gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in its gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases.In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene.
Molecular Weight 58 kDa
Gene ID 11200
NCBI Accession NP_665861, NM_145862
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 514 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-Checkpoint Kinase 2 (CHEK2) (N-Term) antibody WB Suggested Anti-CHEK2 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: Jurkat cell lysate
anti-Checkpoint Kinase 2 (CHEK2) (N-Term) antibody (2) Immunohistochemistry with Placenta tissue at an antibody concentration of 10µg/ml using anti-CHEK2 antibody (ARP30290_P050)
Product cited in: Yomo, Hongo, Kuroyanagi et al.: "Parkinsonism and midbrain dysfunction after shunt placement for obstructive hydrocephalus." in: Journal of clinical neuroscience : official journal of the Neurosurgical Society of Australasia, Vol. 13, Issue 3, pp. 373-8, 2006 (PubMed).

Background publications Ribeiro-Silva, Moutinho, Moura et al.: "Expression of checkpoint kinase 2 in breast carcinomas: correlation with key regulators of tumor cell proliferation, angiogenesis, and survival." in: Histology and histopathology, Vol. 21, Issue 4, pp. 373-82, 2006 (PubMed).

General Kobayashi, Lin: "Antiangiogenic and radiotherapy for cancer treatment." in: Histology and histopathology, Vol. 21, Issue 10, pp. 1125-34, 2006 (PubMed).

Validation Images
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