CHEK2 antibody (N-Term)

Catalog No. ABIN309588

Cited in 1 publication. The Rabbit Polyclonal anti-CHEK2 antibody (ABIN309588) specifically detects CHEK2 in WB and IHC. The antibody is reactive with Human samples.

Quick Overview for CHEK2 antibody (N-Term) (ABIN309588)

Target: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHEK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-CHEK2 Antibody

Binding Specificity: N-Term

Sequence: MSRESDVEAQ QSHGSSACSQ PHGSVTQSQG SSSQSQGISS SSTSTMPNSS

Predicted Reactivity: Human: 100%

Characteristics: This is a rabbit polyclonal antibody against CHEK2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human CHEK2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 514 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-CHEK2 antibody (ABIN309588)

Yomo, Hongo, Kuroyanagi, Kobayashi: "Parkinsonism and midbrain dysfunction after shunt placement for obstructive hydrocephalus." in: Journal of clinical neuroscience : official journal of the Neurosurgical Society of Australasia, Vol. 13, Issue 3, pp. 373-8, (2006) (PubMed).

Target Details for CHEK2

Target: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Alternative Name: CHEK2

Background: In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. CHEK2 is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, CHEK2 is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in its gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in its gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases.In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene.
Alias Symbols: RP11-436C9.1, CDS1, CHK2, HuCds1, LFS2, PP1425, RAD53, hCds1
Protein Interaction Partner: SRPK1, PPP2CA, PLK1, CHEK2, PRKDC, ATM, POLR2L, RCHY1, VHL, UBC, NR4A1, ELAVL1, CDC25A, ARHGAP1, PER3, CDC25C, CUL1, CUL4A, KPNA2, APP, STRAP, TP53, BRCA1, RB1, DAPK3, UBR5, PML, MDM2, BRCA2, KAT2B, LATS2, PSME3, TRIM28, RNF8, STAT1, PPP2R5E, PPP2R5D, PPP
Protein Size: 514

Molecular Weight: 58 kDa

Gene ID: 11200

NCBI Accession: NM_145862, NP_665861

Pathways: p53 Signaling, Apoptosis, Cell Division Cycle