Mitochondrial Ribosomal Protein S15 (MRPS15) (C-Term) antibody

Details for Product No. ABIN309960
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Antigen
Synonyms MPR-S15, RPMS15, S15mt, Mprs15, 1500003E24Rik, 2410002B11Rik, CG4207, DmMRPS15, Dmel\\CG4207, MRP-S15, mRpS15, zgc:92862
Epitope
C-Term
(4), (2), (2), (1), (1)
Reactivity
Human
(13), (5), (4), (2)
Host
Rabbit
(13)
Clonality
Polyclonal
Application
Western Blotting (WB)
(12), (5), (3), (3), (1)
Pubmed 1 reference available
Catalog no. ABIN309960
Quantity 100 µg
Price
229.00 $   Plus shipping costs $45.00
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  • +1 888 205 9894 (TF)
Immunogen Synthetic peptide directed towards the C terminal of human MRPS15
Sequence RRFVTKKALCIRVFQETQKLKKRRRALKAAAAAQKQAKRR NPDSPAKAIP
Predicted Reactivity Human : 100 %
Characteristics This is a rabbit polyclonal antibody against MRPS15. It was validated on Western Blot using a cell lysate as a positive control.
Purification Protein A purified
Alternative Name MRPS15
Background Mammalian mitochondrial ribosomal proteins help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. MRPS15 is a 28S subunit protein that belongs to the ribosomal protein S15P family. The protein is more than two times the size of its E. coli counterpart, with the 12S rRNA binding sites conserved. Between human and mouse, the protein is the least conserved among small subunit ribosomal proteins.Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75 % protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S15P family. The encoded protein is more than two times the size of its E. coli counterpart, with the 12S rRNA binding sites conserved. Between human and mouse, the encoded protein is the least conserved among small subunit ribosomal proteins. Pseudogenes corresponding to this gene are found on chromosomes 15q and 19q.
Molecular Weight 30 kDa
Gene ID 64960
NCBI Accession NM_031280, NP_112570
UniProt P82914
Research Area Chromatin and Nuclear Signaling, DNA/RNA
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 257 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 100 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-Mitochondrial Ribosomal Protein S15 (MRPS15) (C-Term) antibody WB Suggested Anti-MRPS15 Antibody Titration: 2.5ug/ml
ELISA Titer: 1:62500
Positive Control: 721_B cell lysate
Background publications Zhang, Gerstein: "Identification and characterization of over 100 mitochondrial ribosomal protein pseudogenes in the human genome." in: Genomics, Vol. 81, Issue 5, pp. 468-80, 2003 (PubMed).

Hosts (13)
Reactivities (13), (5), (4), (2)
Applications (12), (5), (3), (3), (1)
Epitopes (4), (2), (2), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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