SFPQ antibody (Middle Region)

Catalog No. ABIN310051

This Rabbit Polyclonal antibody specifically detects SFPQ in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Horse, Guinea Pig, Rabbit and Zebrafish (Danio rerio).

Quick Overview for SFPQ antibody (Middle Region) (ABIN310051)

Target: SFPQ (Splicing Factor Proline/glutamine-Ric (SFPQ))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Guinea Pig, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SFPQ antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-SFPQ Antibody

Binding Specificity: Middle Region

Sequence: PVIVEPLEQL DDEDGLPEKL AQKNPMYQKE RETPPRFAQH GTFEYEYSQR

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 92%

Characteristics: This is a rabbit polyclonal antibody against SFPQ. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human SFPQ

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 707 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for SFPQ

Target: SFPQ (Splicing Factor Proline/glutamine-Ric (SFPQ))

Alternative Name: SFPQ

Background: SFPQ is DNA- and RNA binding protein. It is essential pre-mRNA splicing factor required early in spliceosome formation and for splicing catalytic step II, probably as an heteromer with NONO. It binds to pre-mRNA in spliceosome C complex, and specifically binds to intronic polypyrimidine tracts. It interacts with U5 snRNA. It may be involved in a pre-mRNA coupled splicing and polyadenylation process as component of a snRNP-free complex with SNRPA/U1A. SFPQ may be involved in homologous DNA pairing. SFPQ is involved in transcriptional regulation. It binds the DNA sequence 5'-CTGAGTC-3' in the insulin-like growth factor response element (IGFRE) and inhibits IGF-I-stimulated transcriptional activity.
Alias Symbols: POMP100, PSF
Protein Interaction Partner: SNRPA, PTBP1, NONO, HDAC2, FUS, HUWE1, UBC, SUMO2, MDM2, STAU1, RPA3, RPA2, RPA1, ERG, ASB18, ASB12, WWOX, ASB3, EED, rev, TIMM23B, APBB1, PIN1, WBP4, TCERG1, GAS7, PRPF40A, PARK2, UBD, CD81, PAN2, RNF43, WHSC1, VCAM1, SMAD5, ITGA4, FN1, CSNK2A1, BTK, DNA
Protein Size: 707

Molecular Weight: 78 kDa

Gene ID: 6421

NCBI Accession: NM_005066, NP_005057

UniProt: P23246