SLC12A1 antibody (N-Term)

Catalog No. ABIN310136

There are 3+ publications for this product available. The Rabbit Polyclonal anti-SLC12A1 antibody is suitable to detect SLC12A1 in samples from Human, Rat, Mouse, Dog, Pig, Rabbit, Guinea Pig, Cow and Horse. It has been validated for WB and IHC.

Quick Overview for SLC12A1 antibody (N-Term) (ABIN310136)

Target: SLC12A1 (Solute Carrier Family 12 (Sodium/potassium/chloride Transporters), Member 1 (SLC12A1))

Reactivity: Human, Rat, Mouse, Dog, Pig, Rabbit, Guinea Pig, Cow, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC12A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-SLC12A1 Antibody

Binding Specificity: N-Term

Purpose: SLC12A1 Antibody - N-terminal region

Sequence: NEKKSRGFFN YQASIFAENF GPRFTKGEGF FSVFAIFFPA ATGILAGANI

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against SLC12A1. It was validated on Western Blot and immunohistochemistry.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human SLC12A1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8112055

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-SLC12A1 antibody (ABIN310136)

Yoshioka, Kawaguchi, Nishimura, Akagi, Fujisawa, Yanagisawa, Matsumura, Tohyama: "Polyuria-associated hydronephrosis induced by xenobiotic chemical exposure in mice." in: American journal of physiology. Renal physiology, Vol. 311, Issue 4, pp. F752-F762, (2016) (PubMed).

Stec, Drummond, Gousette, Storm, Abraham, Csongradi: "Expression of heme oxygenase-1 in thick ascending loop of henle attenuates angiotensin II-dependent hypertension." in: Journal of the American Society of Nephrology : JASN, Vol. 23, Issue 5, pp. 834-41, (2012) (PubMed).

Kelsen, Patel, Parker, Vera, Rimoldi, Gadepalli, Drummond, Stec: "Heme oxygenase attenuates angiotensin II-mediated superoxide production in cultured mouse thick ascending loop of Henle cells." in: American journal of physiology. Renal physiology, Vol. 295, Issue 4, pp. F1158-65, (2008) (PubMed).

Target Details for SLC12A1

Target: SLC12A1 (Solute Carrier Family 12 (Sodium/potassium/chloride Transporters), Member 1 (SLC12A1))

Alternative Name: SLC12A1

Background:

Background Information: The sodium-potassium-chloride cotransporter isoform 2 is kidney-specific and is found on the apical membrane of the thick ascending limb of Henle's loop and the macula densa. It accounts for most of the NaCl resorption with the stoichiometry of 1Na:1K:2Cl and is sensitive to such diuretics as furosemide and bumetanide. Some Bartter-like syndromes result from defects in this gene.

Gene Name: Solute carrier family 12 (sodium/potassium/chloride transporters), member 1

Alternative Symbols: BSC1, NKCC2

Protein Name: SLC12A1 protein EMBL AAH40138.1

Molecular Weight: 47kDa

Gene ID: 6557

UniProt: Q8IUN5