NADH Dehydrogenase (Ubiquinone) 1 beta Subcomplex, 5, 16kDa (NDUFB5) (Middle Region) antibody

Details for Product No. ABIN310669
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Antigen
Synonyms hm:zeh0024, zgc:123331, 0610007D05Rik, AU015782, SGDH, CISGDH
Epitope
Middle Region
(3), (2), (1), (1), (1)
Reactivity
Mouse (Murine), Rat (Rattus), Cow (Bovine), Xenopus laevis, Fruit Fly (Drosophila melanogaster), Human, Chicken
(9)
Host
Rabbit
(5), (4)
Clonality
Polyclonal
Application
Western Blotting (WB)
(9), (3), (2), (2), (2), (1)
Pubmed 1 reference available
Quantity 50 µg
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Catalog No. ABIN310669
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Immunogen Synthetic peptide directed towards the middle region of human NDUFB5
Sequence KLLRFYIALT GIPVAIFITL VNVFIGQAEL AEIPEGYVPE HWEYYKHPIS
Predicted Reactivity Human : 100 %, African clawed frog : 90 %, Bovine : 90 %, Chicken : 80 %, Mouse : 80 %, Rat : 80 %
Characteristics This is a rabbit polyclonal antibody against NDUFB5. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name NDUFB5
Background NDUFB5 is a subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I). Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.The protein encoded by this gene is a subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I). Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Molecular Weight 17 kDa
Gene ID 4711
NCBI Accession NP_002483, NM_002492
UniProt O43674
Research Area Cancer, Metabolism, Organelles
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 189 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-NADH Dehydrogenase (Ubiquinone) 1 beta Subcomplex, 5, 16kDa (NDUFB5) (Middle Region) antibody WB Suggested Anti-NDUFB5 Antibody Titration: 0.2-1 ug/ml
Positive Control: HepG2 cell lysate
Product cited in: Ton, Hwang, Dempsey et al.: "Identification and primary structure of five human NADH-ubiquinone oxidoreductase subunits." in: Biochemical and biophysical research communications, Vol. 241, Issue 2, pp. 589-94, 1998 (PubMed).

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