UDP-N-Acetyl-alpha-D-Galactosamine:polypeptide N-Acetylgalactosaminyltransferase 10 (GalNAc-T10) (GALNT10) (N-Term) antibody

Details for Product No. ABIN311084
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Antigen
Synonyms AU018154, C330012K04Rik, GalNAc-T10, GalNAc-T9, Galnt9, ppGaNTase, wu:fb37e08, zgc:153114, GALNACT10, PPGALNACT10, PPGANTASE10
Epitope
N-Term
(23), (10), (1), (1), (1)
Reactivity
Mouse (Murine), Rat (Rattus), Zebrafish (Danio rerio), Human
(39), (31), (31), (12), (12), (12)
Host
Rabbit
(36), (2), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(30), (21), (13), (11), (10), (1)
Pubmed 1 reference available
Quantity 50 µg
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Catalog No. ABIN311084
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Immunogen Synthetic peptide directed towards the N terminal of human GALNT10
Sequence VPAGVSLARN LKRVAEVWMD EYAEYIYQRR PEYRHLSAGD VAVQKKLRSS
Predicted Reactivity Chicken : 100 %, Human : 100 %, Mouse : 100 %, Rabbit : 100 %, Bovine : 90 %, Dog : 90 %, Guinea pig : 90 %, Horse : 90 %, Pig : 90 %
Characteristics This is a rabbit polyclonal antibody against GALNT10. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name GALNT10
Background GALNT10 belongs to the polypeptide N-acetylgalactosaminyltransferase (pp-GalNAc-T) family. Polypeptide GalNAc transferases initiate the synthesis of mucin-type oligosaccharides by transferring GalNAc from UDP-GalNAc to the hydroxyl group of either a serine or threonine residue on the polypeptide acceptor. Following expression in insect cells, recombinant GalNAc transferase 10 showed significant GalNAcT activity toward mucin-derived peptides, and it utilized both nonglycosylated and glycosylated peptide substrates.This gene belongs to the polypeptide N-acetylgalactosaminyltransferase (pp-GalNAc-T) gene family. Polypeptide GalNAc transferases initiate the synthesis of mucin-type oligosaccharides by transferring GalNAc from UDP-GalNAc to the hydroxyl group of either a serine or threonine residue on the polypeptide acceptor. Following expression in insect cells, recombinant GalNAc transferase 10 showed significant GalNAcT activity toward mucin-derived peptides, and it utilized both nonglycosylated and glycosylated peptide substrates. Two transcript variants encoding distinct isoforms have been identified for this gene.
Molecular Weight 31 kDa
Gene ID 55568
NCBI Accession NP_060010, NM_017540
UniProt Q86SR1
Research Area Organelles
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 274 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Background publications Cheng, Tachibana, Zhang et al.: "Characterization of a novel human UDP-GalNAc transferase, pp-GalNAc-T10." in: FEBS letters, Vol. 531, Issue 2, pp. 115-21, 2002 (PubMed).

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