SPOP-B antibody (C-Term)

Catalog No. ABIN311288

The Rabbit Polyclonal anti-SPOP-B antibody is suitable to detect SPOP-B in samples from Human, Mouse, Rat, Zebrafish (Danio rerio), Cow, Dog, Guinea Pig, Horse and Rabbit. It has been validated for WB.

Quick Overview for SPOP-B antibody (C-Term) (ABIN311288)

Target: SPOP-B (Speckle-Type POZ Protein (SPOP-B))

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Cow, Dog, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPOP-B antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SPOP-B Antibody

Binding Specificity: C-Term

Purpose: SPOP Antibody - C-terminal region

Sequence: YHASDVLETS GWKSMVVSHP HLVAEAYRSL ASAQCPFLGP PRKRLKQS

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against SPOP. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human SPOP

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8112564

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for SPOP-B

Target: SPOP-B (Speckle-Type POZ Protein (SPOP-B))

Alternative Name: SPOP

Background:

Background Information: SPOP may modulate the transcriptional repression activities of death-associated protein 6 (DAXX), which interacts with histone deacetylase, core histones, and other histone-associated proteins. In mouse, the protein binds to the putative leucine zipper domain of macroH2A1.2, a variant H2A histone that is enriched on inactivated X chromosomes. The BTB/POZ domain of this protein has been shown in other proteins to mediate transcriptional repression and to interact with components of histone deacetylase co-repressor complexes. This gene encodes a protein that may modulate the transcriptional repression activities of death-associated protein 6 (DAXX), which interacts with histone deacetylase, core histones, and other histone-associated proteins. In mouse, the encoded protein binds to the putative leucine zipper domain of macroH2A1.2, a variant H2A histone that is enriched on inactivated X chromosomes. The BTB/POZ domain of this protein has been shown in other proteins to mediate transcriptional repression and to interact with components of histone deacetylase co-repressor complexes. Alternative splicing of this gene results in multiple transcript variants encoding the same protein.

Gene Name: Speckle-type POZ protein

Alternative Symbols: TEF2, BTBD32, NSDVS1, NSDVS2, NEDMACE, NEDMIDF

Protein Name: Speckle-type POZ protein

Molecular Weight: 42kDa

Gene ID: 8405

NCBI Accession: NP_001007227

UniProt: O43791